中国感染控制杂志2025，Vol.24Issue(3)：372-380,9.DOI:10.12138/j.issn.1671-9638.20256645
胃癌术后肺炎患者口咽标本代谢组学研究
Metabolomics of oropharyngeal specimens from postoperative pneumonia patients with gastric cancer
摘要
Abstract
Objective To explore the characteristics of oropharyngeal metabolites of postoperative pneumonia(POP)and non-POP patients with gastric cancer by non-targeted metabolomics,and to provide reference for revea-ling the pathogenesis as well as clinical prevention and control.Methods Oropharyngeal swabs were collected from patients who underwent surgical treatment for gastric cancer in the department of gastrointestinal surgery of a tertia-ry hospital in Fujian Province from January 2022 to December 2023,nested case-control study was adopted,with 30 cases in POP group and non-POP group,respectively,ultrahigh performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)technique was adopted for detection,and multivariate statistical analysis was per-formed.Results The metabolites of POP group and non-POP group preoperatively,POP group and non-POP group postoperatively,POP group preoperatively and postoperatively,as well as non-POP group preoperatively and post-operatively did not show a clear trend of separation on the score graph of principal component analysis(PCA),par-tial least squares discriminant analysis(PLS-DA),and orthogonal partial least squares discriminant analysis(OPLS-DA),but the screening of the differential metabolites revealed that the relative abundances of ascorbic acid and lipoic acid in the POP group preoperatively were lower than non-POP group preoperatively.The relative abundance of 5'-adenylic acid in the POP group preoperatively was lower than postoperatively,while the relative abundances of suc-cinic acid and phenylacetaldehyde were higher.There were differences in metabolic pathways such as phenylalanine.Conclusion Alteration of metabolite level due to abnormalities in specific metabolic pathways presents in the deve-lopment of gastric cancer POP and may be related to the development of POP.关键词
胃癌/术后肺炎/口咽代谢物/非靶向代谢组学Key words
gastric cancer/postoperative pneumonia/oropharyngeal metabolites/non-targeted metabolomics分类
临床医学引用本文复制引用
陈碧贞,李紫梦,蒋雅昕,魏婷妤,贾锐,史超..胃癌术后肺炎患者口咽标本代谢组学研究[J].中国感染控制杂志,2025,24(3):372-380,9.基金项目
福建省自然科学基金计划项目(2021J01875) （2021J01875）
