中国输血杂志2025，Vol.38Issue(3)：327-332,6.DOI:10.13303/j.cjbt.issn.1004-549x.2025.03.005
参麦注射液对西藏高原高血红蛋白人群悬浮红细胞保存质量的影响
Effect of Shenmai injection on the preservation quality of suspended red blood cells prepared from high hemoglobin population in Xizang plateau
摘要
Abstract
Objective To investigate the effect of Shenmai injection on the preservation quality of suspended red blood cells of high hemoglobin population in Xizang plateau.Methods Whole blood(400 mL,n=8)collected by the Xizang Autonomous Region Blood Center was centrifuged at 3 000 g for 10 minutes to remove most of the plasma,followed by the addition of 100 mL of MAP preservation solution to obtain plateau suspended red blood cells,which were then divided into three equal portions.One portion was the control group,and another part had 15 mL of MAP preservation solution added,which was the dosage group.The third portion involved diluting Shenmai injection with MAP,followed by addition of 15 mL of MAP preservation solution containing Shenmai to the red blood cells,resulting in a final concentration of Shenmai injec-tion of 1%,which was the Shenmai group.Blood routine,pH value,electrolytes,glucose,lactate,free Hb,adenosine triphosphate(ATP),P50,phosphatidylserine(PS)and other indicators were detected at day 1,21 and 35,respectively.Results The Hb concentration and Hct of the dosage group and the Shenmai group were significantly lower than those of the control group,with values of(179.3±17.8)vs(181.0±17.1)vs(199.1±19.5)g/L for Hb concentration and(53.2±2.6)%vs(53.3±2.5)%vs(58.4±3.1)%for Hct.The three groups maintained this pattern until the end of storage.In the middle and late stages of preservation,the glucose and Na+contents in the dosage group and the Shenmai group were higher than those in the control group,while the lactate and K+contents were lower than those in the control group.At the end of storage,the glucose and Na+content of the Shenmai group was higher than that of the dosage group,while the lactate and K+content were lower than that of the dosage group.From day 1 to day 35 of storage,the hemolysis rate of the Shenmai group was significantly lower than that of the control group and the dosage group.On day 21 and 35 of storage,the PS ex-pression rate in the Shenmai group was significantly lower than that in the control group and the dosage group,which were(6.52±0.40)%vs(7.24±0.91)%vs(8.27±0.93)%and(7.29±0.53)%vs(9.37±0.82)%vs(8.39±0.76)%,re-spectively.Conclusion The hemolysis rate and PS of suspended red blood cells of Xizang high altitude prepared by adding Shenmai injection were significantly lower than those in the control group and the dosage group,which was conducive to re-ducing hemolysis and slowing down the aging of red blood cells,and had a certain improvement on the preservation quality of suspended red blood cells in Xizang plateau people.关键词
悬浮红细胞/参麦注射液/高海拔地区/血液保存Key words
suspended red blood cell/Shenmai injection/plateau/blood storage分类
医药卫生引用本文复制引用
钟锐,贺曾,王红,李琬晶,刘嘉馨,曹晔..参麦注射液对西藏高原高血红蛋白人群悬浮红细胞保存质量的影响[J].中国输血杂志,2025,38(3):327-332,6.基金项目
四川省中医药管理局科学技术研究专项课题(2023MS540) （2023MS540）
中国医学科学院医学与健康科技创新工程项目"血液安全保障及新产品技术研究"(2021-I2M-1-060) （2021-I2M-1-060）
