中国输血杂志2025，Vol.38Issue(3)：333-342,10.DOI:10.13303/j.cjbt.issn.1004-549x.2025.03.006
负载aPexos和EGCG的可溶微针用于大鼠烫伤伤口愈合的研究
Study on soluble microneedles loaded with aPexos and EGCG for healing of burn wounds in rats
摘要
Abstract
Objective To use Chitosan Methacryloyl(CSMA)loaded with artificial platelet-derived exosomes(aPexos)as the needle body material,and gelatin and carboxymethyl chitosan(CMCS)loaded with Epigallocatechin gal-late(EGCG)as the backing material to prepare microneedles,aims to investigate the therapeutic effect and mechanism of aPexos-EGCG microneedles in the treatment of burn wound healing.Methods First,aPexos were extracted using ultra-sound and gradient ultracentrifugation.The concentration,morphology,and growth factor content(TGF-β1,PDGF-BB,VEGF)of the exosomes were assessed using NTA,transmission electron microscopy,and ELISA kits.The aPexos and EGCG were then dissolved in the needle body and backing materials,respectively.The aPexos and EGCG were loaded into the needle body and backing layer,respectively.The morphology,mechanical properties,and puncture performance of the microneedles were examined,and the preparation conditions for the microneedles loaded with aPexos-EGCG were optimized.Finally,animal experiments and tissue staining were conducted to assess the efficacy of the aPexos-EGCG microneedles in promoting burn wound healing in rats.Results The size distribution of aPexos was mainly in the range of 50-150 nm,with an average diameter of 132.7±3.8 nm and an average concentration of approximately 2.88×1013±3.62×1012 particles/mL.The concentrations of TGF-β1,PDGF-BB,and VEGF were approximately 1 363±135.9 ng/mL,63.5±14.8 ng/mL,and 1 606.0±77.5 pg/mL,respectively.Mechanical property testing of the microneedles indicated that,compared with the blank microneedles,the drug-loaded microneedles had sufficient piercing ability to penetrate the stratum corneum of the skin.The results of the rat burn wound healing experiment showed that the wound healing rates of the d 3,d 7,aPexos-EGCG microneedle group compared to the control group showed significant differences,with respective values of(47.64±12.5)%vs(18.11±6.40)%,(87.45±5.57)%vs(79.85±5.03)%(P<0.05).Hematoxylin-eosin(HE)staining re-vealed more intact epidermal layers in the aPexos-EGCG microneedle group.Masson staining showed that the collagen depo-sition percentage in the aPexos-EGCG microneedle group was higher than in the control group.Immunofluorescence staining results indicated that the aPexos-EGCG microneedle group had a reduced content of M1-type macrophages and an increased content of M2-type macrophages.Additionally,the vascular markers CD31 and α-SMA showed elevated expression in this group,with significant differences compared to the other groups(P<0.05).Conclusion The aPexos-EGCG microneedle developed in this study exhibits good mechanical properties,capable of successfully penetrating the skin′s stratum corneum and delivering aPexos and EGCG.aPexos promotes wound repair,while EGCG regulates the ratio of M1 and M2 macropha-ges at the wound site and suppresses the inflammatory response.This microneedle can effectively promote wound healing in rats with burn injuries,offering a novel approach for tissue repair.关键词
人工血小板外泌体/表没食子儿茶素没食子酸酯/烫伤创面/可溶微针/组织修复Key words
artificial platelet-derived exosomes(aPexos)/Epigallocatechin gallate(EGCG)/burn wound/soluble mi-croneedles/tissue repair分类
医药卫生引用本文复制引用
张阿龙,李琬晶,王红,刘嘉馨,曹晔,钟锐..负载aPexos和EGCG的可溶微针用于大鼠烫伤伤口愈合的研究[J].中国输血杂志,2025,38(3):333-342,10.基金项目
中国医学科学院医学与健康科技创新工程项目"血液安全保障及新产品新技术研究"(2021-I2M-1-060) （2021-I2M-1-060）
