中国输血杂志2025，Vol.38Issue(3)：408-414,7.DOI:10.13303/j.cjbt.issn.1004-549x.2025.03.016
血小板添加液ⅢM悬浮的血小板保存期质量的研究
Platelet quality during storage of platelet concentrates in Platelet Addictive Solution ⅢM:a comparative study
摘要
Abstract
Objective To use Platelet Additive Solution Ⅲ M to suspend concentrated platelets and evaluate their qual-ity at different storage periods,in order to investigate the optimal ratio of Ⅲ M to plasma in the medium for storing concen-trated platelets.Methods Disposable plastic blood bags with platelet storage bags were used to collect whole blood from healthy voluntary blood donors,and concentrated platelets were collected by plasma-rich method,with a volume of about 50 mL and≥4.0×1010 platelets contained in each bag.According to the Platelet Addictive Solution ⅢM/plasma volume ratio in the medium of suspended platelets,the platelets were divided into 3 groups:control group(plasma only),experimental group 1(Platelet Addictive Solution ⅢM/plasma volume ratio of 6.5∶3.5)and experimental group 2(low plasma group,Platelet Addictive Solution ⅢM/plasma volume ratio of 9∶1),each group of 50 samples.Three groups of platelets were stored at(22±2)℃at a flat-bed shaker,and 5 mL were sampled by sterile connection at day 1,3,5 and 7 respectively to detect platelet count,pH value,lactate dehydrogenase,CD62P positive rate and Annexin V positive rate.All the data were analyzed with SPSS24.0 software.One-way ANOVA was employed to compare the differences among three groups.In order to pairwise comparisons between means of multiple samples,Bonferroni method was applied.Results With the extension of storage time,the platelet count decreased and the Annexin V positive rate increased in the 3 groups,and the difference of the 3 groups was not statistically significant(P>0.05).The pH value decreased in the 3 groups,with values at day 1,3,5 and 7 of 7.44±0.13 vs 7.44±0.14 vs 7.41±0.11,7.31±0.68 vs 7.43±0.23 vs 7.22±0.12,7.30±0.15 vs 7.42±0.14 vs 7.17±0.12,7.29±0.33 vs 7.26±0.18 vs 7.04±0.12,respectively.The pH decline in the control group and experi-ment group 1 was minor,with no statistically significant difference,but experiment group 2 showed relatively larger decrea-ses in day 5 and day 7,with statistically significant difference(P<0.05).LDH concentrate was elevated in 3 groups(mmol/L),with values at day 1,3,5 and 7 of 169.62±99.33 vs 105.80±150.71 vs 77.14±105.38,225.10±112.86 vs 116.00±72.77 vs 94.42±88.74,249.42±79.55 vs 119.00±53.51 vs 118.35±80.39,253.34±86.95 vs 147.71±90.71 vs 124.68±128.68 respectively.Compared with the control group,the difference was statistically significant(P<0.05).Experimen-tal group1 had the smallest increase;CD62P positive rate increased in 3 groups(%),with values at day 1,3,5 and 7 of 26.22±11.74 vs 23.48±12.48 vs 40.49±11.86,41.29±8.36 vs 33.53±25.64 vs 50.42±22.36,59.59±10.13 vs 36.39±23.10 vs 50.94±20.50,72.92±15.44 vs 55.54±23.65 vs 61.89±18.82 respectively.Compared with the control group,the increase in experiment group1 was smaller,and the difference was statistically significant(P<0.05).Conclusion Platelet Addictive Solution ⅢM/plasma volume ratio of 6.5∶3.5 is superior to traditional plasma in maintaining platelet quality during the in vitro preservation period of platelets.关键词
血小板保存液ⅢM/血小板质量/CD62P阳性率Key words
Platelet Addictive Solution ⅢM/platelet quality/CD62P positive rate分类
基础医学引用本文复制引用
刘宇健,游冉冉,林绮文,何博,聂咏梅..血小板添加液ⅢM悬浮的血小板保存期质量的研究[J].中国输血杂志,2025,38(3):408-414,7.基金项目
广州市科技计划项目(2023A03J0544) （2023A03J0544）
