中国实验动物学报2025，Vol.33Issue(2)：190-203,14.DOI:10.3969/j.issn.1005-4847.2025.02.004
Padi2敲除加重社会隔离下小鼠的抑郁样行为
Padi2 knockout exacerbates depressive-like behaviors in socially isolated mice
摘要
Abstract
Objective To explore the impact of peptidylarginine deiminase 2(Padi2)-knockout on depressive-like behaviors in socially isolated mice.Methods Using CRISPR/Cas9 technology,a Padi2-knockout(Padi2-/-)mouse model with a C57BL/6J background was established,and the effect of Padi2 knockout was identified by genotyping and RT-qPCR detection.Six-week-old male Padi2-/- mice and wild-type C57BL/6J mice were selected and divided into normal rearing and social isolation groups,with 15 mice per group.The normal rearing group mice were housed with 5 mice per cage,and the social isolation group was housed with 1 mouse per cage,and weighed once a week.After 4 weeks,forced swimming and open field tests were conducted.After the behavioral experiments,brain tissues were taken from mice in each group,and changes in microglia in the brains were detected by immunofluorescence.Results We successfully established Padi2-/- mice.There was no difference in behavior between Padi2-/- mice and C57BL/6J mice in the normal rearing group.After social isolation,compared with C57BL/6J mice,Padi2-/- mice showed a significant increase in depressive symptoms,obvious weight gain,and a significant increase in the number of microglia in brain tissue.Conclusions Padi2 knockout exacerbated depressive-like behaviors and obesity in socially isolated mice,indicating that Padi2 is involved in the progression of depression and may be an effective target for the prevention and treatment of depression.关键词
抑郁样行为/肽基精氨酸脱亚胺酶2/社会隔离/基因敲除/小胶质细胞Key words
depressive-like behavior/peptidylarginine deiminase 2/social isolation/gene knockout/microglia分类
生物学引用本文复制引用
赵亚,吕冲昊,罗世帆,刘可,郑泽民,白冰,师长宏..Padi2敲除加重社会隔离下小鼠的抑郁样行为[J].中国实验动物学报,2025,33(2):190-203,14.基金项目
陕西省创新能力支持计划(2022PT-36,2022PT-42),军队实验动物专项课题(SYDW2020-20),国家自然青年科学基金(32300428). Funded by Shaanxi Province Innovation Capacity Support Plan(2022PT-36,2022PT-42),Special Topic of Military Experimental Animals(SYDW2020-20),National Natural Science Foundation of China(NSFC)Young Scientists Fund Project(32300428). （2022PT-36,2022PT-42）
