中国实验动物学报2025，Vol.33Issue(2)：216-224,9.DOI:10.3969/j.issn.1005-4847.2025.02.006
2,4-二硝基氯苯诱导BALB/c荷瘤小鼠特应性皮炎模型的建立
Establishment of an atopic dermatitis model in tumor-bearing BALB/c mice induced by 2,4-dinitrochlorobenzene
摘要
Abstract
Objective In this study,2,4-dinitrochlorobenzene(DNCB)was used to induce the establishment of an atopic dermatitis(AD)model in BALB/c homozygous mice to simulate the skin inflammatory complications in patients with clinical malignancies.Methods BALB/c mice were divided into different groups:negative control group(NC group),model group(MODEL group),atopic dermatitis group(AD group),and dexamethasone group(DEX group).After the mice in MODEL group and DEX group were inoculated with S-180 tumor cells in the axilla,MODEL group,AD group and DEX group were stimulated with DNCB on the dorsal skin and the ear to establish an animal model of atopic dermatitis in tumor-bearing mice.Changes in body weight were observed and recorded,the dorsal skin condition of mice was assessed after the last administration of the drug,the spleen was taken to calculate the spleen coefficient,the difference in the mass of mouse ear slices was determined to calculate the degree of auricular swelling and the rate of inhibition of swelling,and histopathological tests were performed on the dorsal skin tissues to detect the levels of IgE,TNF-α,IL-4,and IL-17 in the serum using an ELISA assay.Results Compared with the NC group,the skin of mice in the MODEL and AD groups showed erythematous,papular,scaly and mossy changes,accompanied by weight loss,and a significant increase in splenic coefficient and auricular swelling.Pathologic findings showed an incomplete skin structure,a significant increase in skin thickness,a large infiltration of inflammatory cells,and an increase in the number of mast cells.Serum levels of IgE,TNF-α,IL-4 and IL-17 were increased.Compared with the MODEL group,the DEX group showed an improvement in all the assays.Conclusions DNCB excitation can successfully establish an animal model of AD in hormonal mice,which is drug-controllable,which provides a useful scientific tool for conducting scientific research related to malignant tumors and skin inflammation.关键词
动物模型/特应性皮炎/恶性肿瘤/BALB/c小鼠Key words
animal model/atopic dermatitis/malignant tumor/BALB/c mice分类
生物学引用本文复制引用
牛晓涛,左佳倩,黄玉洁,任雯沁,束昕妍,舒鹏..2,4-二硝基氯苯诱导BALB/c荷瘤小鼠特应性皮炎模型的建立[J].中国实验动物学报,2025,33(2):216-224,9.基金项目
江苏省中医药科技发展计划重点项目(ZD202214),国家自然科学基金(82374539),江苏省研究生实践创新计划(KYCX24_2230). Funded by the Jiangsu Province Chinese Medicine Science and Technology Development Program Key Project(ZD202214),National Natural Science Foundation of China(82374539),Jiangsu Province Graduate Student Practice and Innovation Program(KYCX24_2230). （ZD202214）
