中国实验动物学报2025，Vol.33Issue(2)：241-248,8.DOI:10.3969/j.issn.1005-4847.2025.02.009
电针对糖尿病神经痛大鼠背根神经节TRPV4表达的影响
Effect of electroacupuncture on the expression of TRPV4 in the dorsal root ganglion of diabetic neuropathic pain model rats
摘要
Abstract
Objective To explore the function of electroacupuncture(EA)on body mass,fasting blood glucose,heat pain threshold,and transient receptor potential vanilloid 4(TRPV4)in the dorsal root ganglia(DRG)of rats with diabetic neuropathic pain(DNP).Methods A DNP rat model was formed by intraperitoneally injecting the animals with STZ.From days 15 to 21,bilateral Zusanli and Kunlun points of the DNP rat model were treated with electroacupuncture once daily for 30 min.We then measured their body mass,fasting blood glucose,and heat pain threshold.The co-expression of TRPV4 and NeuN in the rat L4～L6 DRG was detected by immunofluorescence.The effects of the TRPV4 agonist GSK1016790A on body mass,fasting blood glucose,and the heat pain threshold of DNP rats treated with electroacupuncture were detected.Results After the 7th day,body mass was significantly decreased(P<0.01)and fasting glucose was significantly increased(P<0.01)in the model group compared with the normal group.After the 21st day,compared with the model group,heat pain threshold of the model+electroacupuncture group was significantly higher(P<0.01);the results of co-expression of TRPV4 and NeuN immunofluorescence on rat L4～L6 DRG showed that:the expression of positive cells in the model group was significantly higher(P<0.01)than that in the normal group,the co-expression of TRPV4 and NeuN positive cells in L4～L6 DRG of rats in the model+electroacupuncture group was significantly lower(P<0.01)than that in the model group.The TRPV4 agonist GSK1016790A can reverse the downregulation of thermal pain threshold induced by electroacupuncture in DNP rats(P<0.01).Conclusion Electroacupuncture alleviated the DNP induced by STZ,and its mechanism may involve the inhibition of TRPV4 protein expression in the DRG.关键词
糖尿病神经痛/电针/背根神经节/瞬时受体电位香草蛋白4Key words
diabetic neuropathic pain/electroacupuncture/dorsal root ganglia/transient receptor potential vanilloid 4分类
生物学引用本文复制引用
江民健,方剑乔,何晓芬,池恒愉,康玉蓉,蒋永亮,郑崯沐,李斯苡,周姝婷,刘伯宇,邵晓梅..电针对糖尿病神经痛大鼠背根神经节TRPV4表达的影响[J].中国实验动物学报,2025,33(2):241-248,8.基金项目
浙江省自然科学基金项目(ZCLY24H2701,LY22H270006),国家中医药管理局科技司-浙江省共建项目重点项目(GZY-ZJ-KJ-24073),浙江中医药大学附属医院专项(2022FSYYZZ09,2024FSYYZY05). Funded by the Natural Science Foundation of Zhejiang Province(ZCLY24H2701,LY22H270006),Key Projects of Science and Technology Department of State Administration of Traditional Chinese Medicine-Zhejiang Province Co-Construction Project(GZY-ZJ-KJ-24073),Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine Special(2022FSYYZZ09,2024FSYYZY05). （ZCLY24H2701,LY22H270006）
