中国药物评价2025，Vol.42Issue(1)：28-34,7.
鹿茸提取物抑制心肌肥厚的作用机制研究
The Effect of Deer Antler Extract on Inhibiting Cardiac Hypertrophy
摘要
Abstract
Objective:Given that cardiac hypertrophy is an important risk factor for heart failure and research in the field of traditional Chinese medicine on cardiac hypertrophy remains insufficient,this study is dedicated to exploring the potential efficacy and mechanism of action of deer antler extract in inhibiting cardiac hypertrophy,especially its possible targets.Methods:In this study,the RNA sequen-cing gene sets of hearts from sham-operated and cardiac hypertrophy mice were mined using the GEO database.The target genes of deer antler were obtained through the Herb website,and differential gene pathway enrichment analysis was performed using the DAVID plat-form.Meanwhile,functional enrichment analysis of differential genes was carried out using Metascape.To verify the effect of deer antler extract,an Ang Ⅱ-induced cardiomyocyte hypertrophy model was constructed.The area of cardiomyocytes was evaluated by immunofluo-rescence,and the expression of common genes among the myocardial hypertrophy markers BNP,β-MHC,the target genes of deer antler,and the differential genes of myocardial hypertrophy was detected by qRT-PCR.Results:The gene set GSE2459 was successfully mined from the GEO database,and there were four common genes between this gene set and the target genes of deer antler.Among them,3 genes showed up-regulated expression in the myocardial hypertrophy model,while 1 gene was down-regulated.The experimental results in-dicated that deer antler extract could effectively inhibit the increase in the area of cardiomyocytes induced by Ang Ⅱ and simultaneously reduce the mRNA expression levels of BNP and β-MHC in cardiomyocytes.In addition,deer antler extract could also down-regulate the mRNA expression of ATP13A3,NPY5R and HSD17B7,and up-regulate the mRNA expression of CRISP3.Conclusion:Deer antler ex-tract can inhibit Ang Ⅱ-induced myocardial hypertrophy,and its potential mechanism may be related to the regulation of the expression of common target genes ATP13A3,NPY5R,HSD17B7 and CRISP3.This can provide a basis for the application of deer antler in the treat-ment of myocardial hypertrophy,hoping to open up a new direction for the research on the prevention and treatment of myocardial hypertro-phy by traditional Chinese medicine.关键词
心肌肥厚/鹿茸/RNA测序/差异基因/共有基因Key words
Cardiac hypertrophy/Deer antler/RNA sequencing/Differentially expressed genes/Shared genes分类
中医学引用本文复制引用
李海艳,刘芮,崔悦,任婧,鄂明尧,赵磊..鹿茸提取物抑制心肌肥厚的作用机制研究[J].中国药物评价,2025,42(1):28-34,7.基金项目
吉林省中医药科技项目(2023167):鹿茸多肽通过Nrf2/HO-1信号通路改善H9C2心肌细胞肥大的机制研究 （2023167）
