中国肿瘤生物治疗杂志2025，Vol.32Issue(2)：169-175,7.DOI:10.3872/j.issn.1007-385x.2025.02.005
靶向趋化因子配体1对口腔鳞状细胞癌细胞恶性生物学行为的影响
Effects of targeting chemokine ligand 1 on the malignant biological behaviors of oral squamous cell carcinoma cells
摘要
Abstract
Objective:To explore the expression of chemokine ligand 1(CCL1)in oral squamous cell carcinoma(OSCC)and its effect on the proliferation,migration and invasion of human oral tongue squamous cell carcinoma cells(HSC-4).Methods:28 OSCC tissue samples and clinical characteristic data of patients were collected at the Affiliated Stomatological Hospital of Southwest Medical University between January 2018 and June 2020,as well as 10 normal gingival tissue samples removed during the extraction of impacted teeth.OSCC cells HSC-4 were cultured routinely and divided into the control group(without virus),the NC group(transfected with control lentiviral vector),the shCCL1 group(transfected with knockdown CCL1 lentiviral vector),and the CCL1 group(culture medium containing 60 ng/mL CCL1 recombinant protein).Immunohistochemistry and WB were used to detect the expression of CCL1 in OSCC tissues and cells,and analyze the correlation between its expression level and the clinical features of patients.qPCR,CCK-8 assay,plate cloning assay,cell scratch test,Transwell assay and flow cytometry were used to detect the expression of CCL1 mRNA,the proliferation,migration and invasion abilities and the apoptosis of HSC-4 cells respectively.Results:CCL1 protein was highly expressed in OSCC tissues and HSC-4 cells(all P<0.01)and its expression was related to the clinical stage of tumors(P<0.05).The expression of CCL1 in HSC-4 cells was successfully knocked down(P<0.000 5).Knocking down the expression of CCL1 could inhibit the proliferation(P<0.05 or P<0.01),migration and invasion(all P<0.05)of HSC-4 cells,and promote its apoptosis(all P<0.05).CCL1 recombinant protein treatment resulted in the opposite effects(P<0.05,P<0.01,P<0.000 1).Conclusion:CCL1 is highly expressed in OSCC and its expression is correlated with the clinical stage of OSCC.CCL1 may take part in regulating the proliferation,migration,invasion and apoptosis of HSC-4 cells.关键词
口腔鳞状细胞癌/HSC-4细胞/趋化因子配体1(CCL1)/增殖/迁移/侵袭/凋亡Key words
oral squamous cell carcinoma(OSCC)/HSC-4 cell/chemokine ligand 1(CCL1)/proliferation/migration/invasion/apoptosis分类
临床医学引用本文复制引用
李晶莹,许译文,刘梦琳,聂敏海,刘旭倩..靶向趋化因子配体1对口腔鳞状细胞癌细胞恶性生物学行为的影响[J].中国肿瘤生物治疗杂志,2025,32(2):169-175,7.基金项目
国家自然科学基金项目(No.82401112) （No.82401112）
四川省科技厅自然科学(重点、面上)项目(No.2025ZNSFSC0760) （重点、面上）
大学生创新创业训练计划项目(No.S202410632135) （No.S202410632135）
