中山大学学报（自然科学版）（中英文）2025，Vol.64Issue(2)：67-75,9.DOI:10.13471/j.cnki.acta.snus.ZR20240099
UFLC-QTOF-MS/MS分析噁唑酰草胺及其在生物体内的代谢产物
Analysis of matamifop and its metabolic products in biological systems via UFLC-QTOF-MS/MS
摘要
Abstract
Utilizing ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UFLC-QTOF-MS/MS)technology,and employing the larvae of mealworm(Tenebrio molitor)as a model organism,this study developed a new approach to discover and accumulate chromatographic-mass spectrometric characteristic data of metamifop metabolites in the absence of comprehensive metabolite standards.The metabolic pathway of metamifop in T.molitor was further elucidated.The study identified 21 metabolites in the excreta of mealworms larvae that were fed a diet containing 2 mg/g of metamifop over a period of 15 days.These included 14 phase I metabolites and 7 phase Ⅱ metabolites.Among the phase I metabolites,beside the reported compounds such as Metamifop monooxide,Fenoxaprop acid,6-CBO,HPFMPA,HPPA,and HFMPA,eight were identified for the first time in any biological system.It was also observed that these phase I metabolites underwent transformation into corresponding phase Ⅱ metabolites through three primary pathways:Conjugation with amino acids,glucose-sulfate esterification,and sulfation.关键词
噁唑酰草胺(metamifop)/黄粉虫(Tenebrio molitor)/代谢产物/超高效液相色谱-四极杆串联飞行时间质谱(UFLC-QTOF-MS/MS)Key words
metamifop/Tenebrio molitor/metabolite/UFLC-QTOF-MS/MS分类
生物学引用本文复制引用
罗剑文,钟创光,刘羿飞,黄欣如,黄昆,彭霏,殷梓宁,何昀泽,张以顺..UFLC-QTOF-MS/MS分析噁唑酰草胺及其在生物体内的代谢产物[J].中山大学学报（自然科学版）（中英文）,2025,64(2):67-75,9.基金项目
广东省高等教育学会实验室管理专业委员会基金(GDJ20220159) （GDJ20220159）
中山大学校级教学质量与教学改革工程类项目(33000-12220011) （33000-12220011）
