作物学报2025，Vol.51Issue(4)：863-872,10.DOI:10.3724/SP.J.1006.2025.44134
天山雪莲质膜水孔蛋白基因SiPIP1;3在番茄中的抗寒功能分析
Functional analysis of the plasma membrane intrinsic protein gene SiPIP1;3 from Saussurea involucrata in tomato
摘要
Abstract
Aquaporins(AQPs),as key facilitators of water transport across cellular membranes,play an essential role in plant growth and adaptation to environmental stresses.Previously,the plasma membrane intrinsic protein gene SiPIP1;3 was cloned from a low-temperature expression library of Saussurea involucrata,a cold-tolerant herbaceous plant.To investigate the function of SiPIP1;3 under low-temperature stress,a plant expression vector containing SiPIP1;3 was constructed and transformed into cold-sensitive tomato plants.The results demonstrated that SiPIP1;3 expression significantly enhanced tomato tolerance to low-temperature treatment by promoting the accumulation of soluble proteins,soluble sugars,and proline,while reducing mem-brane lipid peroxidation.Moreover,field cultivation results revealed that SiPIP1;3 expression improved intercellular CO2 concen-tration by increasing stomatal conductance in tomato leaves.This led to a marked improvement in net photosynthetic efficiency and water use efficiency,ultimately resulting in a significant increase in both the average fruit size and fruit number per plant.In conclusion,the expression of SiPIP1;3 significantly enhances low-temperature tolerance and fruit yield in tomato plants.This study provides a valuable genetic resource and establishes a theoretical foundation for breeding cold-resistant tomato varieties.关键词
SiPIP1/3基因/抗寒性/光合效率/果实产量Key words
SiPIP1/3 gene/cold resistance/photosynthetic efficiency/fruit yield引用本文复制引用
张晓丽,刘晓燕,夏雯雯,李锦..天山雪莲质膜水孔蛋白基因SiPIP1;3在番茄中的抗寒功能分析[J].作物学报,2025,51(4):863-872,10.基金项目
本研究由国家自然科学基金项目(32260726),"天池英才"引进计划项目(CZ001617)和石河子大学高层次人才科研启动项目(RCZK202470)资助. This study was supported by the National Natural Science Foundation of China(32260726),the Tianchiyingcai Foundation(CZ001617),and the Shihezi University High Level Talent Research Launch Project(RCZK202470). （32260726）
