作物学报2025，Vol.51Issue(4)：888-899,12.DOI:10.3724/SP.J.1006.2025.44156
甘蓝型油菜角果长度和每角粒数基因定位以及角果皮转录组动态分析
Mapping of silique length and seeds per silique and transcriptome profiling of pod walls in Brassica napus L.
摘要
Abstract
Rapeseed(Brassica napus L.)is a major oilseed crop globally,and improving yield remains a primary objective in rapeseed breeding programs.Yield in rapeseed is determined by three main components:siliques per unit area,seeds per silique,and seed weight.Although silique length is not a direct yield component,it influences both seeds per silique and seed weight,and thus indirectly affects yield.In this study,two parental lines with contrasting silique lengths and seeds per silique,YA and Zhong-shuang 11,along with their 211 recombinant inbred lines(RILs),were used as experimental materials.The RIL population was genotyped through genome resequencing and grown in two environments:autumn in Changsha and summer in Mingle.Silique length and seeds per silique were measured,and quantitative trait loci(QTL)analysis was conducted.The results identified a major QTL for both silique length and seeds per silique on chromosome A09 in both environments.Comparative RNA-seq analy-sis of pod walls from the two parents,conducted 3-21 days after flowering,indicated that genes involved in photosynthesis,plant hormone signaling transduction,and secondary metabolite biosynthesis play critical roles in pod wall development.Among the differentially expressed genes,BnaA09.CYP78A9,BnaC08.SCL13,and BnaA04.ARL,which are associated with auxin response and signaling transduction,were identified as candidate genes regulating silique length.These findings provide a foundation for fine mapping and exploring the regulatory mechanisms of genes controlling silique length in rapeseed,which could contribute to yield improvement in breeding programs.关键词
甘蓝型油菜/角果长度/每角粒数/数量性状位点/转录组分析Key words
Brassica napus L/silique length/seeds per silique/quantitative trait location(QTL)/transcriptome profiling引用本文复制引用
王晓琳,刘忠松,康雷,杨柳..甘蓝型油菜角果长度和每角粒数基因定位以及角果皮转录组动态分析[J].作物学报,2025,51(4):888-899,12.基金项目
本研究由国家重点研发计划项目(2022YFD1200400)资助. The study was supported by the National Key Research and Development Program of China(2022YFD1200400). （2022YFD1200400）
