作物学报2025，Vol.51Issue(4)：900-913,14.DOI:10.3724/SP.J.1006.2025.42040
水稻转录因子OsERF104的克隆和功能研究
Cloning and functional analysis of OsERF104 transcription factor in rice
摘要
Abstract
Ethylene Responsive Factor(ERF),a subfamily of the APETALA2/Ethylene Responsive Factor(AP2/ERF)family,plays critical roles in regulating diverse biological processes,including plant growth and development,hormone signaling,and responses to abiotic stresses.Investigating the functions of the ERF family in rice(Oryza sativa L.)provides valuable genetic resources for rice breeding.In this study,the OsERF104 gene(LOC_Os08g36920)was cloned.Bioinformatic analysis revealed that the full-length coding sequence of OsERF104 is 849 bp,encoding a protein of 283 amino acids.OsERF104 contains a con-served domain characteristic of the AP2/ERF family and shares the highest sequence similarity with the AtERF96 protein in Arabidopsis thaliana,which is known to be involved in salt tolerance.Subcellular localization analysis confirmed that the Os-ERF104 protein is localized in the nucleus,indicating that it functions as a nuclear transcription factor.Cis-acting element analy-sis of the OsERF104 promoter identified elements associated with hormone responses,abiotic stress,and light responses.To examine the role of OsERF104 under abiotic stress,its expression pattern was analyzed using RT-qPCR.OsERF104 was ex-pressed in various rice tissues,with the highest expression observed in the leaf sheath.Its expression was downregulated by ABA and GA but upregulated by JA,PEG,and NaCl treatments.Transcriptional activation assays showed that the full-length and C-terminal fragments of OsERF104 exhibit transcriptional activity,while the N-terminal fragment and the AP2 domain alone do not.Transgenic rice lines of overexpressing or knocking out OsERF104 were generated via genetic transformation.Phenotypic analysis demonstrated that OsERF104-overexpressing rice exhibited enhanced sensitivity to ABA and increased tolerance to salt stress during the seedling stage compared with the wild-type ZH11.In contrast,oserf104 mutant rice displayed the opposite phe-notypes.In conclusion,OsERF104 positively regulates salt tolerance in rice.This study provides a strong foundation for further exploration of the biological functions and molecular mechanisms of OsERF104 in rice.关键词
水稻/OsERF104/转录因子/盐胁迫/表达分析/亚细胞定位Key words
rice/OsERF104/transcription factor/salt stress/expression analysis/subcellular localization引用本文复制引用
潘炬忠,韦萍,朱德平,邵胜雪,陈珊珊,韦雅倩,高维维..水稻转录因子OsERF104的克隆和功能研究[J].作物学报,2025,51(4):900-913,14.基金项目
本研究由广西自然科学基金青年科学基金项目(2024GXNSFBA010324,2025GXNSFAA069066)和国家自然科学基金青年科学基金项目(32301749)资助. This study was supported by the Young Scientists Fund of Guangxi Natural Science Foundation(2024GXNSFBA010324,2025GXNSFAA069066)and the Young Scientists Fund of the National Natural Science Foundation of China(32301749). （2024GXNSFBA010324,2025GXNSFAA069066）
