作物学报2025，Vol.51Issue(4)：914-931,18.DOI:10.3724/SP.J.1006.2025.44123
基于种子发育转录组的豌豆Bzip基因家族分析及种子发育候选基因的鉴定
Analysis of bZIP gene family and identification of seed development candidate genes in pea based on seed development transcriptome
摘要
Abstract
The bZIP gene is widely involved in physiological processes such as seed development,light signal regulation,and stress response.However,little is known about the regulation of seed development by pea PastbZIP gene.In order to identify the PastbZIP gene family related to pea seed development and reveal its evolutionary relationship,this study used bioinformatics methods and seed development transcriptome data to identify and analyze the PastbZIP gene family,and mined the candidate PastbZIP genes related to seed development through comparative genomics,at the same time,it was verified by protein inter-action network and qRT-PCR.A total of 62 PastbZIP genes were identified from the transcriptome data of pea seed develop-ment,and PastbZIP genes were divided into 9 groups according to phylogenetic characteristics,Different subfamilies showed evolutionary diversity and differences in protein physicochemical properties,gene structure,conserved motifs,etc.Collinearity analysis showed that pea had 54,23,8,and 22 pairs of collinearity gene with soybean,Medicago truncatula,Arabidopsis and faba bean,respectively.More important,it was found that the fragment replication was the main driving force for the expansion of the PastbZIP gene family,and the evolution of these genes was achieved through purification selection.KEGG enrichment analysis showed that PastbZIP was mainly enriched in plant hormone signal transduction pathways.The same is that the cis-acting ele-ments of the PastbZIP promoter contain a large number of hormone response elements,and 60 PastbZIP(96.8%)genes contained five hormone response elements.Ten candidate genes related to seed development were found in pea by homologous comparison,and protein interaction genes also regulated seed development.In conclusion,this study was the first to identify and analyze the members of the PastbZIP family related to seed development in pea,and excavated the candidate genes related to seed develop-ment through homologous alignment.These results will provide important reference for the study of PastbZIP gene regulation of seed development.关键词
豌豆/PastbZIP/基因家族/种子发育/进化分析/蛋白互作网络Key words
pea/PastbZIP/gene family/seed development/evolutionary analysis/protein interaction network引用本文复制引用
周恩强,石宇欣,李波,汪凯华,顾春燕,王学军,魏利斌,缪亚梅,周瑶,姚梦楠,赵娜,王永强,朱宇翔,薛冬,李宗迪..基于种子发育转录组的豌豆Bzip基因家族分析及种子发育候选基因的鉴定[J].作物学报,2025,51(4):914-931,18.基金项目
本研究由财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-08-Z10),江苏省农业科技自主创新项目[CX(22)2011]和江苏省"双创人才"项目(YSSCRC2022469)资助. This study was supported by the China Agriculture Research System of MOF and MARA(CARS-08-Z10),the Independent Innovation Pro-ject of Agricultural Science and Technology in Jiangsu Province[CX(22)2011],and the Jiangsu Province"Double Talents"Project(YSSCRC2022469). （CARS-08-Z10）
