作物学报2025，Vol.51Issue(4)：932-942,11.DOI:10.3724/SP.J.1006.2025.41042
彩色青稞和彩色小麦籽粒的代谢组学比较分析
Comparative analysis of metabolomics of colored hulless barley and colored wheat grains
摘要
Abstract
Colored hulless barley and wheat are valuable and unique germplasm resources,with nutrient composition and content varying among grains of different colors.In this study,metabolomic analysis based on LC-MS was applied to grains from two colored hulless barley varieties and two wheat varieties at different days after pollination,including Ehei 720135(EH720135),Guangzhou Blue Barley(GZL),Zhongke Zimai(ZKZM),and Nongda 4218 Zi(ND4218).A total of 936 metabolites were de-tected across the 23 samples,comprising 379 known substances and 557 unknowns.Cluster analysis and PCA revealed significant differences in the metabolite profiles of grains at different developmental stages.Most metabolites exhibited dynamic changes throughout grain development.In total,687 metabolites showed differential abundances between the mature seeds of the four varieties,of which 206 were significantly different between the two hulless barley varieties and the two wheat varieties.Further-more,308 differential metabolites were identified between the two-colored wheat varieties,and 277 between the two colored hulless barley varieties.Further analysis revealed that metabolites related to flavonoids,such as catechins,flavonoid derivatives,and chrysoeriol,were significantly more abundant in mature hulless barley grains compared to colored wheat grains,suggesting that these may be specific functional components of hulless barley.This study not only enhances our understanding of the meta-bolic differences between hulless barley and wheat,but also provides a theoretical foundation for the development of functional foods using colored hulless barley and colored wheat.关键词
LC-MS/彩色青稞/彩色小麦/代谢组Key words
LC-MS/colored hulless barley/colored wheat/metabolome引用本文复制引用
程红娜,徐得泽,董静,秦丹丹,许甫超,徐晴,彭严春,孙龙清,徐乐,郭英,杨新泉..彩色青稞和彩色小麦籽粒的代谢组学比较分析[J].作物学报,2025,51(4):932-942,11.基金项目
本研究由财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-05),优质青贮大麦新品种选育及冬闲田应用关键技术研发(2023BBB100)和功能作物新种质创制及其食品研发和功效评价(2022ZZCX006)资助. This study was supported by the China Agriculture Research System of MOF and MARA(CARS-05),the Research and Development of the Key Technology for Breeding and Application of Elite Silage Barley Varieties in Winter Fallow Field(2023BBB100),and the Creation,Application and Evaluation of New Germplasms of Functional Crops(2022ZZCX006). （CARS-05）
