作物学报2025，Vol.51Issue(4)：958-968,11.DOI:10.3724/SP.J.1006.2025.43055
基于RNA-seq和PER-seq联合分析探究ZmHDZ6表达调控网络
Integrative analysis of RNA-seq and PER-seq to elucidate regulatory network of ZmHDZ6 expression
摘要
Abstract
Maize is a crop with high water demand,and drought is a major factor limiting its productivity.Building on previous findings and related research,we identified that the ZmHDZ6 gene is strongly induced by drought,and transgenic plants overex-pressing ZmHDZ6 exhibit enhanced drought resistance.To investigate the downstream regulatory mechanisms of the maize tran-scription factor ZmHDZ6,RNA-seq was performed on transgenic maize plants overexpressing ZmHDZ6,while PER-seq(proto-plast transient expression-based RNA sequencing)was conducted using protoplasts from the B73 inbred line.An integrative analysis of these datasets revealed that the differentially expressed genes(DEGs)identified by both methods showed consistent results in GO analysis,with their functions primarily associated with redox reactions.KEGG pathway analysis further demon-strated consistency in the benzoxazinoid biosynthesis pathway.Notably,RNA-seq DEGs were additionally enriched in amino acid and nucleotide metabolism pathways,while PER-seq DEGs were enriched in ribosome biogenesis pathways.Based on these findings,we propose that ZmHDZ6 enhances drought resistance in maize by regulating genes involved in redox processes and benzoxazinoid metabolism.Furthermore,through an integrative analysis of DNA binding motifs of the HD-ZIP I family and the promoters of 129 common DEGs(Co-DEGs),combined with gene annotation and motif physical location information,we nar-rowed potential target genes down to 16,of which 8 are closely associated with stress responses in maize.This study provides a detailed analysis of the regulatory network of ZmHDZ6 expression,offering valuable insights into the drought resistance mecha-nisms mediated by ZmHDZ6 and a reference for further functional studies.关键词
RNA-seq/PER-seq/转录因子/ZmHDZ6/靶基因/motifKey words
RNA-seq/PER-seq/transcription factor/ZmHDZ6/target gene/motif引用本文复制引用
方应浩,周波,陈茹梅,杨文竹,秦慧民..基于RNA-seq和PER-seq联合分析探究ZmHDZ6表达调控网络[J].作物学报,2025,51(4):958-968,11.基金项目
本研究由国家自然科学基金项目(32372064)资助. This study was supported by the National Natural Science Foundation of China(32372064). （32372064）
