花生荚果产量相关性状QTL定位与候选基因分析
QTL mapping and candidate gene analysis of peanut pod yield-related traits
摘要
Abstract
Peanut(Arachis hypogaea L.)is an important economic and oilseed crop in China,with pod traits playing a critical role in determining yield.In this study,a recombinant inbred line(RIL)population derived from a cross between the large-pod local variety"Dongguan Banman(DB)"and the small-pod variety"ZLA"was used to construct a high-density genetic map with single nucleotide polymorphism(SNP)markers.This map was employed to identify quantitative trait loci(QTL)associated with pod traits across four distinct cultivation environments.A total of 30 QTLs were mapped to chromosomes A01,A03,A05,A06,A07,A08,B02,B04,B06,and B10,with logarithm of odds(LOD)values ranging from 4.04 to 34.17 and contribution rates from 3.10%to 33.52%.Among these,13 major QTLs were associated with pod length,width,thickness,and 100-pod weight,showing LOD values between 4.41 and 34.17 and contribution rates between 11.21%and 33.52%.Notably,qPLA07 was consistently de-tected across all four environments,while qPWA08.1,qPWB02,and qPTB06 were stable in three environments.Additionally,14 epistatic QTLs were identified,with LOD values ranging from 5.07 to 6.67 and phenotypic variation explained(PVE)from 4.21%to 21.84%.KEGG pathway enrichment analysis of genes within the QTL regions of qPWA08.1,qPWB02,and qPTB06 identified four candidate genes:Ahy_A08g039622,Ahy_B02g057642,Ahy_B06g085859,and Ahy_B06g085890,based on gene functional annotation and expression analysis across peanut tissues.These findings provide a theoretical foundation for identifying key genes regulating peanut pod yield and for developing molecular markers to facilitate breeding programs.关键词
花生/荚果/QTL/候选基因Key words
peanut/pod/QTL/candidate genes引用本文复制引用
林伟津,李少雄,鲁清,郭泽佳,刘浩,李海芬,王润风,黄璐,余倩霞,陈小平,洪彦彬..花生荚果产量相关性状QTL定位与候选基因分析[J].作物学报,2025,51(4):969-981,13.基金项目
本研究由国家自然科学基金项目(32172051,32301869),财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-13),国家重点研发计划项目(2023YFD1202800),省级乡村振兴战略专项资金种业振兴项目(2024),广东省重点领域研发计划项目-现代种业(2022B0202060004)和广东省基础与应用基础研究基金面上项目(2023A1515010098,2024A1515010511)资助. This study was supported by the National Natural Science Foundation of China(32172051,32301869),the China Agriculture Research Sys-tem of MOF and MARA(CARS-13),the National Key R&D Program of China(2023YFD1202800),the Special Funds for the Revitalization of Agriculture through Seed Industry under the Provincial Rural Revitalization Strategy(2024),the Guangdong Provincial Key Research and Development Program-Modern Seed Industry(2022B0202060004),and the Guangdong Basic and Applied Basic Research Foundation(2023A1515010098,2024A1515010511). （32172051,32301869）
