作物学报2025，Vol.51Issue(4)：982-991,10.DOI:10.3724/SP.J.1006.2025.44147
Ⅰ型MADS-box基因SlMADS79调控番茄株型的功能研究
Functional study on the regulation of plant architecture by tomato type Ⅰ MADS-box gene SlMADS79
摘要
Abstract
MADS-box genes play a crucial role in regulating plant growth and development.While the functions of type Ⅱ MADS-box genes have been extensively studied,there are relatively few reports on type Ⅰ MADS-box genes in tomato.In this study,we cloned the type Ⅰ MADS-box gene SlMADS79,which was found to be highly expressed in tomato roots,leaves,and lateral buds,suggesting its involvement in the regulation of vegetative organ growth.Using the classical tomato cultivar Ailsa Craig(AC++)as background material,we silenced the SlMADS79 gene through RNA interference(RNAi).Compared to the wild type,SlMADS79-silenced lines exhibited reduced apical dominance and decreased plant height.The length,width,perimeter,and area of the leaves were smaller than those of wild-type plants.Additionally,root traits—including total length,total surface area,total projected area,volume,number of forks,and number of tips—were significantly reduced.Anatomical studies revealed that while the cells in the longitudinal sections of SlMADS79-silenced stems were smaller,their average number significantly in-creased.At the hormonal level,the contents of IAA(indole-3-acetic acid),TZR(trans-zeatin riboside),and CS(castasterone)were decreased in the SlMADS79-silenced lines.At the molecular level,the auxin response gene IAA3 and gibberellin synthesis gene GA3ox1 were significantly downregulated in the SlMADS79-silenced lines,while the cell cycle gene CyCA3;1 was significantly upregulated.This study further analyzes the biological function of the SlMADS79 gene at morphological,anatomical,hormonal,and molecular levels,expands our understanding of the type Ⅰ MADS-box gene family in tomato,and provides a solid theoretical foundation for further research on plant architecture regulation in tomato.关键词
番茄/MADS-box/SlMADS79/株高/叶片形态/根系Key words
tomato/MADS-box/SlMADS79/plant height/leaf morphologies/root system引用本文复制引用
郭绪虎,李灵芝,李凤,马博岩,贾晓宇..Ⅰ型MADS-box基因SlMADS79调控番茄株型的功能研究[J].作物学报,2025,51(4):982-991,10.基金项目
本研究由山西省基础研究计划项目(202303021211181),大同市应用基础研究计划项目(2024067)和山西大同大学基础研究项目(2022Q3)资助. This study was supported by the Basic Research Programs of Shanxi Province(202303021211181),the Applied Basic Research Program of Datong City(2024067),and the Basic Research Programs of Shanxi Datong University(2022Q3). （202303021211181）
