作物学报2025，Vol.51Issue(4)：1061-1076,16.DOI:10.3724/SP.J.1006.2025.41054
CO2浓度升高、升温及其交互作用对良星99冬小麦叶片碳氮代谢的影响
Effects of elevated CO2 concentration,increased temperature and their in-teraction on the carbon and nitrogen metabolism in Liangxing 99 winter wheat leaves
摘要
Abstract
It is well established that either elevated atmospheric CO2 concentration or increased temperature alone can signifi-cantly influence wheat growth and yield.However,limited research has explored the combined effects of elevated CO2 and in-creased temperature on wheat throughout its entire growth period.In this study,winter wheat(Triticum aestivum"Liangxing99")was cultivated in environment-controlled chambers under two CO2 concentrations(ambient and ambient+200 µmol mol-1 and two temperature regimes(ambient and ambient+2).Phenological development,photosynthesis,carbohydrate metabolism,℃nitrogen assimilation,and yield were systematically investigated.Elevated CO2 enhanced the net photosynthetic rate and water use efficiency during the elongation,anthesis,and grain filling stages,while also increasing soluble sugar content during the grain filling stage.Although the activities of glutamine synthetase(GS)and glutamic-pyruvic transaminase(GPT)decreased during grain filling,elevated CO2 still increased biomass and yield by 32.8%and 30.0%,respectively,primarily by increasing the number of grains.In contrast,increased temperature shortened the overall growth period of winter wheat,reduced glutamate synthetase(GOGAT)activity during the elongation stage,and decreased soluble sugar,starch,and sucrose contents.Additionally,increased temperature lowered water use efficiency and GOGAT activity at anthesis,as well as sucrose synthase and GS activities during grain filling,resulting in a 12.2%reduction in biomass,though yield was not significantly affected.Elevated CO2 mitigated the adverse effects of increased temperature by advancing flowering,extending the grain filling period,and alleviating the inhibition of photosynthesis and nitrogen assimilation.Specifically,elevated CO2 upregulated TaRUBP1 expression during elongation,which enhanced the net photosynthetic rate and increased soluble sugar content at elongation and sucrose content at anthesis.Moreover,elevated CO2 upregulated TaGS2 expression at anthesis and TaNR expression during grain filling,which increased GS activity at anthesis and nitrate reductase(NR)activity during grain filling.These responses alleviated the inhibition of nitrogen assimilation caused by high temperature.Consequently,elevated CO2 mitigated the negative effects of increased temperature on biomass production,while enhancing yield by 23.9%under elevated temperature,primarily through an increased number of grains.In summary,elevated CO2 alleviated the negative impacts of increased temperature on winter wheat biomass by enhancing photosynthetic capacity,promoting the accumulation of photosynthetic assimilates,improving nitrogen assimilation,and extending the grain filling period.Additionally,it increased yield by boosting the number of grains under elevated temperature conditions.关键词
冬小麦/碳氮代谢/生长/产量/CO2浓度升高/温度升高Key words
winter wheat/carbon and nitrogen metabolism/growth/yield/elevated CO2 concentration/increased temperature引用本文复制引用
王娇,郝兴宇,白海霞,韩语燕,梁惠,冯雅楠,张东升,李萍,宗毓铮,史鑫蕊..CO2浓度升高、升温及其交互作用对良星99冬小麦叶片碳氮代谢的影响[J].作物学报,2025,51(4):1061-1076,16.基金项目
本研究由山西农业大学青年拔尖创新人才支持计划项目(BJRC201602)和山西农业大学农学院研究生教育改革与质量提升工程顶目(2023YDT03,2023YCX05,2023YCX42)资助. This study was supported by the Youth Talents Support Program of Shanxi Agricultural University(BJRC201602)and the Graduate Education Reform and Quality Improvement Program of College of Agriculture,Shanxi Agricultural University(2023YDT03,2023YCX05,2023YCX42). （BJRC201602）
