植物研究2025，Vol.45Issue(2)：181-190,10.DOI:10.7525/j.issn.1673-5102.2025.02.004
植物热形态建成中PIF4调控SAURs基因表达
PIF4 Regulates the Expression of SAURs in Plant Thermomorphogenesis
摘要
Abstract
Thermomorphogenesis is defined as a series of morphological changes that occur in higher plants when the temperature is higher than an optimal temperature and lower than the stress temperature.Among these morphological changes,hypocotyl elongation induced by high temperature has received the most attention and has been studied most deeply.SMALL AUXIN UP RNA(SAUR)gene family plays a key role in promoting growth and cell elongation.However,little is known about the molecular mechanism of high temperature regulation of SAURs.In this study,Arabidopsis Col-0 wild type,pif4-2 mutant and four transgenic plants were used as research materials.Real-time Quantitative PCR(qPCR),chromatin immunoprecipitation(ChIP),double luciferase reporter gene detection and phenotypic analysis were used to explore the molecular mechanisms of high temperature regulated SAUR1-SAUR4.The results were as follows:high temperature promoted SAUR1-SAUR4 transcription and this promotion required transcription factor PHYTOCHROME-INTERACTING FACTOR 4(PIF4);transcription factor PIF4 binded to the E-box region in the promoter regions of SAUR1-SAUR4,and mild-high temperature enhanced these binding;auxin was downstream of PIF4 in the thermomorphogenesis signaling pathway;auxin signaling pathway was required for the regulation of SAUR1-SAUR4 by PIF4.These results indicated that high temperature regulated the transcription of these four genes by influencing the binding strength of PIF4 and SAUR1-SAUR4 promoter chromatin.This research deepens our understanding of the molecular mechanism underlying hypocotyl elongation downstream genes regulated by high temperature during thermomorphogenesis,thus enriching the theoretical basis of heat-resistant breeding.关键词
热形态建成/下胚轴伸长/PIF4/SAURsKey words
thermomorphogenesis/hypocotyl elongation/PIF4/SAURs分类
生物学引用本文复制引用
王玺尧,刘艳玲,王昕..植物热形态建成中PIF4调控SAURs基因表达[J].植物研究,2025,45(2):181-190,10.基金项目
国家自然科学基金项目(31600220). （31600220）
