中药药理与临床2025，Vol.41Issue(2)：27-32,6.
基于UPLC-Q-TOF-MS技术的通脉养心丸入血、入心脏组织成分分析及其质谱裂解规律研究
UPLC-Q-TOF-MS Reveals Components of Tongmai Yangxin(通脉养心丸)Pills in Blood and Heart and Their Fragmentation Patterns
摘要
Abstract
Objective:In this study,ultra-high performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS)was employed to rapidly and qualitatively analyze the components of Tongmai Yangxin(通脉养心丸)pills in the serum and cardi-ac tissue samples of rats.Methods:The Waters ACQUITY UPLC BEH C18 column was used and MS scanning was carried out in positive and negative ion modes.The MS information such as data acquisition mode,adduct ion in MS1,collision energy in MS2,and fragment in MS2 was used to identify the compounds.Results:A total of 17 compounds were identified from the serum samples of rats treated with Tongmai Yangx-in pills,including 9 flavonoids,4 saponins,3 pentacyclic triterpenoids,and 1 organic acid.A total of 3 compounds were identified from the heart tissue samples,including 2 flavonoids and 1 saponin.Conclusion:This study analyzed the original and metabolized components of Tong-mai Yangxin pills in the blood and heart to reveal the changes and transmission processes of chemical components in vivo,providing an experi-mental basis for the quality control,clinical application,and new drug development of Tongmai Yangxin pills.关键词
通脉养心丸/超高效液相色谱-四极杆-飞行时间质谱法/定性分析/体内化学成分Key words
Tongmai Yangxin(通脉养心丸)pills/Ultra-high performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry/Qualitative analysis/Chemical components in vivo引用本文复制引用
唐艳,陈婷,刘思琦..基于UPLC-Q-TOF-MS技术的通脉养心丸入血、入心脏组织成分分析及其质谱裂解规律研究[J].中药药理与临床,2025,41(2):27-32,6.基金项目
国家自然科学基金资助项目(编号:82405131) （编号:82405131）
中药粉体与创新药物省部共建国家重点实验室培育基地开放基金(编号:23PTKF1019) （编号:23PTKF1019）
组分中药国家重点实验室资助课题(编号:CBCM2024103) （编号:CBCM2024103）
高层次人才科研启动基金(编号:1101/0001020) （编号:1101/0001020）
国家自然科学基金预研项目(编号:22023YYJJ04) （编号:22023YYJJ04）
湖南中医药大学本科生科研创新基金项目(编号:2023BKS029). （编号:2023BKS029）
