中药药理与临床2025，Vol.41Issue(2)：54-59,79,7.
肉桂醛缓解高糖诱导的SH-SY5Y细胞铁死亡的作用研究
Cinnamaldehyde Alleviates High Glucose-Induced Ferroptosis in SH-SY5Y Cells
摘要
Abstract
Objective:To investigate the protective effect of cinnamaldehyde on diabetes-related ferroptosis in neurocytes.Methods:The injury of mature SH-SY5Y neuronal cells was triggered by high glucose,and then the cells were treated with cinnamaldehyde(13.2 and 26.4 mmol/L)and lipstatin-1(0.01 mmol/L),respectively.The cell viability was determined by the MTT assay,and the reactive oxygen species(ROS)level in cells was observed by fluorescence microscopy.Flow cytometry was employed to determine the intracellular Fe2+level and mitochon-drial membrane potential(MMP).The morphological changes of mitochondria were observed by transmission electron microscopy.The pro-tein levels of transferrin receptor(TFRC),ferritin heavy chain 1(FTH1),and solute carrier family 7 member 11(SLC7A11)were deter-mined by Western blotting.Results:Compared with normal cells,the SH-SY5Y cells exposed to high glucose showed decreased viability,in-creased intracellular ROS level,decreased MMP and glutathione(GSH)/oxidized glutathione(GSSG)ratio,elevated Fe2+level,and downreg-ulated expression levels of negative regulatory proteins of ferroptosis.Compared with the cells exposed to high glucose,cinnamaldehyde treat-ment improved the cell viability(P<0.05),lowered the intracellular ROS level(P<0.01),increased the MMP and GSH/GSSG ratio(P<0.05 or P<0.01),and lowered the Fe2+level(P<0.05).In addition,cinnamaldehyde ameliorated high glucose-induced aberrations such as mitochondrial atrophy and breakage and disappearance of mitochondrial cristae,upregulated the expression levels of FTH1 and SLC7A11(negative regulators of ferroptosis),and downregulated the protein level of TFRC(P<0.01).Conclusion:Cinnamaldehyde alleviates high glucose-induced ferroptosis by modulating the iron metabolism and inhibiting cellular iron-dependent lipid peroxidation.关键词
肉桂醛/神经损伤/高糖/铁死亡/转铁蛋白/铁蛋白重链/轻链亚基溶质载体家族 7 成员 11/线粒体/谷胱甘肽/谷胱甘肽过氧化酶Key words
Cinnamaldehyde/Nerve damage/High glucose/Ferroptosis/TFRC/FTH1/SLC7A11/GSH/GSH-Px引用本文复制引用
陈秀婷,杨莹莹,王佳琪,周金玲,夏星..肉桂醛缓解高糖诱导的SH-SY5Y细胞铁死亡的作用研究[J].中药药理与临床,2025,41(2):54-59,79,7.基金项目
国家自然科学基金项目(编号:81960728) （编号:81960728）
广西高校中青年教师科研基础能力提升项目(编号:2022KY0302) （编号:2022KY0302）
广西中医药大学博士科研启动基金项目(编号:2021BS007) （编号:2021BS007）
广西中医药大学桂派杏林拔尖人才项目(编号:2022C007) （编号:2022C007）
广西中医药大学桂派中医药传承创新团队项目(编号:2022B005) （编号:2022B005）
广西中医药大学研究生教育创新计划项目(编号:YCSZ2022006) （编号:YCSZ2022006）
广西中医药大学青年创新研究团队项目(编号:2015QT002). （编号:2015QT002）
