中华中医药学刊2025，Vol.43Issue(3)：42-46,后插3-后插9,12.DOI:10.13193/j.issn.1673-7717.2025.03.007
基于中红外光谱技术结合HPLC对不同产地地黄快速无损鉴别
Rapid and Nondestructive Identification of Dihuang(Rehmannia glutinosa)of Different Origins Based on Infrared Spectroscopy and High Performance Liquid Chromatography
摘要
Abstract
Objective To carry out the rapid and nondestructive identification of Dihuang(Rehmannia glutinosa)of different origins.Methods The infrared absorption spectrum of a total of 166 batches of Dihuang(Rehmannia glutinosa)in 16 counties and districts in four provinces was collected for differential analysis,and the results of the analysis were verified by high performance liquid chromatography.Results The infrared spectra of the 166 samples were basically similar in peak shape,with the same ab-sorption at 2928,1643,1419,1353,1238,1145,1053,925,799,700～543 cm-1.The differences between samples from the three sites in Henan Province were mainly at 1519～1494 cm-1.The differences between samples from different sites in Hebei Prov-ince were mainly at 1571 and 1020 cm-1.The differences between samples from different sites in Shanxi Province were mainly at 1790～1642,1440,1030 cm-1.Then among the differences between provinces,Shandong Province was significantly different from other provinces mainly at 1796,1718,1640,1606～1567,1492,1453,1277,1024 cm-1.Among of them,the main charac-teristic component in the band of 1640～1439 cm-1 was catalpol,and the strong absorption peak at 1052 cm-1 was C-O bond stretching vibration absorption in the structure of pistil side.Conlusion Infrared spectroscopy combined with HPLC can provide i-deas for the rapid and non-destructive identification of different origins of Dihuang(Rehmannia glutinosa).关键词
地黄/中红外光谱/高效液相色谱法/不同产地/聚类分析Key words
Dihuang(Rehmannia glutinosa)/mid-infrared spectroscopy/HPLC/different origins/clustering analysis分类
医药卫生引用本文复制引用
乔璐,阴紫钰,段浩瀚,谢赢赢,徐可可,刘永红,李菊香,董诚明,王磊..基于中红外光谱技术结合HPLC对不同产地地黄快速无损鉴别[J].中华中医药学刊,2025,43(3):42-46,后插3-后插9,12.基金项目
国家重点研发计划项目(2017YFC1702800) （2017YFC1702800）
河南省重大科技专项项目(171100310500) （171100310500）
河南省科技攻关基金项目(222102310182) （222102310182）
河南省大学生创新创业训练计划项目(202410471017) （202410471017）
河南中医药大学博士科研基金项目(BSJJ2014-01) （BSJJ2014-01）
