低温处理下日本医蛭及其环带组织转录组差异表达基因分析OA

[Objective]This study aimed to investigate the effects of low temperature on the expression of related genes in different tissues of Hirudo nippnica.[Method]Forty H.nipponica were randomly divided into two groups,three tissues(cliellum,muscle and head)of H.nippnica were sequenced,assembled and annotated at normal temperature(24℃)and low temperature(6℃)conditions with starvation for 28 days,and the dif-ferentially expressed genes(DEGs)of different tissues were analyzed by GO and KEGG databases,meanwhile,the DEGs were validated by qPCR.[Result]Under different temperatures,there were 2 465,2 588 and 1 998 DEGs in cliellum,muscle and head,respectively,of which 737 DEGs were shared by three tissues.Gene on-tology analysis indicates that the gene expression of related tryptophan metabolism and enzyme activity in bio-logical process and molecular function were significant.KEGG pathway analysis suggests that hypothermia affected many pathways related to energy metabolism(PPAR,AMPK,NF-kappa,BGnRH)and the conversion of unsaturated fatty acids,as well as the expression of related DEGs.LDLR,CaM,notch,Plk1 and ADIPOR genes were up-regulated in the cliellum,which may be involved in the development and regulation of the gonad of H.nippnica.[Conclusion]These differential genes are involved in the metabolism of tryptophan,the trans-formation of fatty acids and the proliferation of germ cells under low temperature treatment,this study underpin understanding the mechanism of low temperature mediated reproduction of H.nippnica.