安徽农业大学学报2025，Vol.52Issue(1)：49-55,7.DOI:10.13610/j.cnki.1672-352x.20250320.002
基于黑腹胃蝇嗅觉行为筛选普氏野马粪便挥发物的初步研究
A preliminary study on the screening of volatiles in Equus przewalskii faeces based on the olfactory behavior of Gasterophilus pecorum(Diptera:Gasterophilidae)
摘要
Abstract
[Objective]Gasterophilus pecorum is the only species of stomach bot fly that lays eggs on pasture and thereby infects the host,and the newly emerged flies often gather near fresh feces of the host.The role of Przewalski's horse(Equus przewalskii)fecal volatiles in the olfactory communication of the adult G.pecorum remains to be explored.[Method]The behavioral responses of G.pecorum to standard compounds of seven fecal volatiles from Przewalski's horse were tested.Compounds with significant responses were screened out,and the selection rate of G.pecorum at different concentrations were determined.In addition,a related function between substance concentration and the olfactory selection rate of G.pecorum was established through nonlinear fitting.[Result](1)Two compounds,acetophenone(P<0.01)and nonanal(P<0.05),were screened out as significantly attractive to females,while no significant effect was observed in males(P>0.05).(2)Females showed the highest response to acetophenone at a lower concentration(10-6 mL·mL-1)and to nonanal at a higher concentration(10-1 mL·mL-1).(3)The olfactory selection curve for nonanal showed a higher fit for females(R²=0.88),while the fit for acetophenone was slightly lower(R²=0.65).[Conclusion]In summary,acetophenone and nonanal present in fecal volatiles play a certain indicative role in olfactory communication of female G.pecorum.Due to the different roles of male and female flies in reproduction,it is speculated that there are olfactory differences between the two genders.These differences are not only manifested in their different preferences for odors in different environ-ments,but also in their different responses to the same compounds.Therefore,when developing control agents,it is possible to specialize in interfering with females only to achieve the purpose of parasitic control.关键词
黑腹胃蝇/普氏野马/粪便挥发物/嗅觉行为/苯乙酮/壬醛Key words
Gasterophilus pecorum/Equus przewalskii/fecal volatile compounds/olfactory behavior/ace-tophenone/nonanal分类
农业科技引用本文复制引用
王安琪,刘佳伟,张钰,赛力克别克·堆山,马伟,田茂贤,李凯..基于黑腹胃蝇嗅觉行为筛选普氏野马粪便挥发物的初步研究[J].安徽农业大学学报,2025,52(1):49-55,7.基金项目
新疆自然保护地调查与国家公园潜力区科学考察项目(2021xjkk1201)和国家林草局野生动植物保护司物种保护项目(2022-HXFWBHQ-LK-03)共同资助. （2021xjkk1201）
