基于黑腹胃蝇嗅觉行为筛选普氏野马粪便挥发物的初步研究OA

[Objective]Gasterophilus pecorum is the only species of stomach bot fly that lays eggs on pasture and thereby infects the host,and the newly emerged flies often gather near fresh feces of the host.The role of Przewalski's horse(Equus przewalskii)fecal volatiles in the olfactory communication of the adult G.pecorum remains to be explored.[Method]The behavioral responses of G.pecorum to standard compounds of seven fecal volatiles from Przewalski's horse were tested.Compounds with significant responses were screened out,and the selection rate of G.pecorum at different concentrations were determined.In addition,a related function between substance concentration and the olfactory selection rate of G.pecorum was established through nonlinear fitting.[Result](1)Two compounds,acetophenone(P<0.01)and nonanal(P<0.05),were screened out as significantly attractive to females,while no significant effect was observed in males(P>0.05).(2)Females showed the highest response to acetophenone at a lower concentration(10-6 mL·mL-1)and to nonanal at a higher concentration(10-1 mL·mL-1).(3)The olfactory selection curve for nonanal showed a higher fit for females(R²=0.88),while the fit for acetophenone was slightly lower(R²=0.65).[Conclusion]In summary,acetophenone and nonanal present in fecal volatiles play a certain indicative role in olfactory communication of female G.pecorum.Due to the different roles of male and female flies in reproduction,it is speculated that there are olfactory differences between the two genders.These differences are not only manifested in their different preferences for odors in different environ-ments,but also in their different responses to the same compounds.Therefore,when developing control agents,it is possible to specialize in interfering with females only to achieve the purpose of parasitic control.