安徽农业大学学报2025，Vol.52Issue(1)：164-172,9.DOI:10.13610/j.cnki.1672-352x.20250320.008
负载β-胡萝卜素的中空介孔SiO2乳液的制备及其酶促反应
Preparation and enzymatic reaction of hollow mesoporous SiO2 emulsion loaded with β-carotene
摘要
Abstract
[Objective]This paper aimed to promote the degradation of β-carotene by carotenoid cleavage di-oxygenases(CCDs)to produce tobacco flavouring cleavage products.[Method]An oil-in-water Pickering emulsion based on the stabilizing effect of solid nanoparticles was constructed in this study.The effects of commercially available mesoporous silica nanoparticles and prepared hollow mesoporous silica nanoparticles on the physicochem-ical properties and microstructures of the β-carotene-loaded Pickering emulsion were investigated at the same con-centration.In order to achieve the contact between carotenoid cracking dioxygenase and β-carotene substrate,Pick-ering emulsion environment was used as a high performance interface catalytic platform to promote the enzymatic reaction,to compare the aroma composition and relative content ratio of substrate after enzymatic hydrolysis reaction in different environments.[Result](1)The internal surface area of HMSNs with hollow structure was 396.361 m2·g-1,the pore capacity was 0.296 cc·g-1,and the average pore size was 5.880 nm,which was 1.9,3.1 and 1.4 times of that of MSNs,respectively.(2)The average particle size of HMSNs/MSNs Pickering emulsion was 30 μm/45 μm,respectively.The former emulsion had smaller drop size,higher residual emulsion volume fraction and more stable emulsion system,which could effectively improve the catalytic activity of carotenoid cracking dioxygenase.(3)In HMSNs Pickering emulsion environment,CCDs cleaved β-carotene to produce 80 tobacco aroma compounds,36 chemical components were increased compared with MSNs emulsion.[Conclusion]This study provides a theoreti-cal basis for the cleavage of β-carotene by carotenoid cleavage dioxygenase in the water-oil interface environment,as well as for the preparation of β-carotene degradation products with improved application effects in cigarettes.关键词
Pickering 乳液/β-胡萝卜素/中空介孔二氧化硅/类胡萝卜素裂解双加氧酶/香气成分Key words
Pickering emulsion/beta-carotene/hollow mesoporous silica/carotenoid cleavage dioxygenase/aroma components分类
轻工业引用本文复制引用
薛雯雯,刁洪林,朱智杰,王文斌,梅丽萍,赵伦旭,杜先锋..负载β-胡萝卜素的中空介孔SiO2乳液的制备及其酶促反应[J].安徽农业大学学报,2025,52(1):164-172,9.基金项目
安徽中烟工业有限责任公司委托项目(202134000034029)资助. （202134000034029）
