安徽农业科学2025，Vol.53Issue(6)：133-138,6.DOI:10.3969/j.issn.0517-6611.2025.06.030
番茄青枯病拮抗菌的筛选鉴定及其复配能力研究
Screening and Identification of Antagonistic Bacteria Against Tomato Bacterial Wilt and Its Compounding Ability
谭泽宝 1桓宝林 1肖琴 1黄文静 1罗坤1
作者信息
- 1. 湖南农业大学植物保护学院,湖南 长沙 410128
- 折叠
摘要
Abstract
[Objective]To screen out the biocontrol bacteria with broad spectrum resistance,and find the hybrid strains that have no resist-ance to each other and amplify the control effect of tomato bacterial wilt.[Method]Strains with antagonistic ability to Rella soleculosa were se-lected from the stem base of healthy tomato plants in Changsha,Hunan Province by using inhibition zone method and AGAR diffusion method,and their classification status was determined by morphological observation,16S rRNA gene sequencing and phylogenetic analysis.More func-tions of biocontrol strains were explored by the determination of extracellular enzyme production capacity and bacteriostatic spectrum.[Result]From the 16 strains with antagonistic ability,two strains of biocontrol bacteria were identified as having high antagonistic ability and showed no difference in combination ability.The screened TB-5 was preliminatively identified as Bacillus mojavensis,and its inhibitory zone size against tomato bacterial wilt was 21.7 mm.XL-7 was identified as Bacillus siamensis,and its inhibitory zone size against tomato bacterial wilt was 23.6 mm.Strains TB-5 and XL-7 had different ability to produce protease and ferriferous carrier.They showed resistance to Phytophthora,Scle-rotium rolfsii,Alternaria alternata,cabbage black spot,Colletotrichum capsici and Sarcophora citri.After the combination of the two strains,the diameter of the inhibition zone of the fermentation liquid was enlarged to 27.0 mm,and the diameter of the inhibition zone of the fermenta-tion supernatant reached 24.5 mm.[Conclusion]Through the investigation of the extracellular enzyme production ability and antibacterial spectrum determination including the combination ability of strains TB-5 and XL-7,the problem of unstable control effect of traditional single biocontrol bacteria has been greatly strengthened,and the potential of biocontrol has been increased.关键词
番茄青枯病/拮抗细菌/复配/产酶能力/抑菌谱Key words
Tomato bacterial wilt/Antagonistic bacteria/Compounding/Enzyme production ability/Bacteriostatic spectrum分类
植物保护学引用本文复制引用
谭泽宝,桓宝林,肖琴,黄文静,罗坤..番茄青枯病拮抗菌的筛选鉴定及其复配能力研究[J].安徽农业科学,2025,53(6):133-138,6.
