重庆医学2025，Vol.54Issue(3)：567-572,6.DOI:10.3969/j.issn.1671-8348.2025.03.002
Sab在性别差异性急性肝损伤小鼠中的作用机制研究
Mechanism of Sab in sexual dimorphism of acute liver injury in mice
摘要
Abstract
Objective To investigate the mechanism of SH3 domain binding protein(Sab)in sex-differ-ential acute liver injury in mice.Methods Female and male mice were randomly divided into control group,acetaminophen(APAP)-induced acute liver injury group,Ad-Sab group,estrogen receptor antagonist group and estrogen receptor agonist group.After corresponding treatments,liver tissues and serum were collected for ALT/AST detection,hematoxylin-eosin(HE)staining and Western blot analysis.Results In the APAP-induced acute liver injury group,male mice showed significantly higher p-JNK activity in hepatic cytoplasm and mitochondria than female mice(P＜0.05).Male mice had lower Sab expression levels than female mice(P＜0.05).In the Ad-Sab group,male mice injected with Ad-Sab showed more severe hepatic injury,higher Sab expression and elevated ALT levels compared with Ad-lacZ injected male mice(P＜0.05).In the estrogen receptor antagonist group,fulvestrant-treated females showed dose-dependent increases in Sab expression.Fe-male mice treated with Ad-Sab plus fulvestrant showed more severe liver injury and higher ALT levels than those receiving Ad-lacZ plus fulvestrant after 4 h of 200 mg/kg APAP administration exhibited more severe liver injury and higher ALT levels than controls(P＜0.05).PPT-protected males in the estrogen receptor ag-onist group showed reduced hepatic damage and lower ALT levels compared with control male mice(P＜0.05).Conclusion Sab expression level determines the severity of JNK-dependent acute drug-induced liver injury.Fe-male mice have lower hepatic Sab protein expression levels and exhibit significant resistance to liver injury.关键词
肝损伤/对乙酰氨基酚/性别差异性/SH3结构域结合蛋白Key words
liver injury/acetaminophen/sex differences/Sab分类
医药卫生引用本文复制引用
蒋逍达,黄旭,张军..Sab在性别差异性急性肝损伤小鼠中的作用机制研究[J].重庆医学,2025,54(3):567-572,6.基金项目
湖北省自然科学基金资助项目(2022CFB112). （2022CFB112）
