摘要
Abstract
This study aims to investigate the effects of enzymatically hydrolyzed complex protein on the growth performance,blood bio-chemical indicators,and intestinal health of piglets.The research evaluates the feasibility of using enzymatically hydrolyzed complex protein as a substitute for fish meal in feed while reducing high-dose zinc oxide,thereby providing a practical application basis for production.A single-factor experimental design was employed,in which 400 piglets approximately 10 kg,aged 36 days were randomly divided into four groups,with five replicates per treatment and 20 pigs per replicate.The experimental period lasted for 25 days.The four treatments included:a control group(basal diet without fish meal,zinc content 1 500 mg/kg),a fish meal group(containing 3%fish meal,zinc content 1 500 mg/kg),a composite protein group(replacing 3%fish meal with 3%enzymatically hydrolyzed composite pro-tein,zinc content 1 500 mg/kg),and a low zinc group(adding 5%enzymatically hydrolyzed composite protein,zinc content 65 mg/kg).The experimental results indicated that the final weight and average daily gain(ADG)of the composite protein group and low zinc group were significantly higher than those of the control group(P<0.05).The diarrhea index and rate for each experimental group were significantly lower than those of the control group(P<0.05),with the composite protein group exhibiting a significantly lower diarrhea rate compared to both the fish meal group and the low zinc group(P<0.05).Blood biochemical indicators revealed that the levels of urea nitrogen(UN)in each experimental group were significantly lower than those in the control group(P<0.05),while alkaline phos-phatase(AKP)levels were significantly higher(P<0.05).Additionally,compared to the control group,the levels of diamine oxidase(DAO)and D-lactate(D-lac)in each experimental group were significantly reduced(P<0.05).In summary,substituting fish meal with equiva-lent proportions of enzymatic complex protein in piglet feed can significantly enhance piglet growth performance,reduce the incidence of diarrhea,and maintain intestinal integrity.Furthermore,incorporating a higher proportion of enzymatic complex protein can decrease the necessity for high doses of zinc oxide.The findings of this study offer a scientific evaluation of the feasibility of replacing fish meal with enzymatic complex proteins and reducing the reliance on high doses of zinc oxide in feed,thereby providing a practical founda-tion for real-world applications.关键词
酶解复合蛋白/仔猪/生长性能/血液生化指标/肠道健康Key words
Enzymatically hydrolyzed complex protein/Piglet/Ggrowth performance/Blood biochemical indicators/Intestinal health
