福建畜牧兽医2025，Vol.47Issue(2)：54-56,3.
鸡柔嫩艾美耳球虫EtIMP1基因的克隆与表达载体的构建
Cloning and construction of expression vector for EtIMP1 gene of chicken Eimeria tenella
廖加磊1
作者信息
- 1. 南平市延平区农业农村局 福建 南平 353000
- 折叠
摘要
Abstract
This study extracted and reverse transcribed RNA from chicken Eimeria tenella,cloned the IMP1 gene,and connect the PCR product to the pEasy-Blunt-Simple vector,identify it correctly by PCR,and send it to the company for sequencing and sequence analysis.Result:The EtIMP1 gene of chicken Eimeria tenella was successfully cloned,with an ORF length of 1 194 bp,and its cor-rectness was verified;Constructing the EtIMP1 gene expression vector pEasy-Blunt-Simple IMP1 and obtaining a recombinant plas-mid,which can provide a technical method for in vitro expression of the IMP1 gene and lay the foundation for screening vaccine can-didate genes.关键词
鸡柔嫩艾美耳球虫/IMP1基因/克隆/表达载体/PCRKey words
Chicken Eimeria tenella/IMP1 gene/Cloning/Expression vector/PCR引用本文复制引用
廖加磊..鸡柔嫩艾美耳球虫EtIMP1基因的克隆与表达载体的构建[J].福建畜牧兽医,2025,47(2):54-56,3.
