南方医科大学学报2025，Vol.45Issue(3)：488-493,6.DOI:10.12122/j.issn.1673-4254.2025.03.06
白纹伊蚊幼虫摄食量影响生长发育及对溴氰菊酯的抗药性
Effects of larval feeding amount on development and deltamethrin resistance in Aedes albopictus
摘要
Abstract
Objective To investigate how larval feeding regimens influence development and deltamethrin resistance of Aedes albopictus to provide evidence for standardizing larval feeding protocols in studies of insecticide resistance.Methods Aedes albopictus larvae of a laboratory resistant strain were divided into 3 groups(n=500)and reared with high,medium,and low food availability(100,50,or 25 mg daily for the 1st and 2nd instars,and 500 mg 250,or 125 mg daily for 3rd and 4th instars).The developmental time,pupation rate,adult emergence rate,adult body weight,and wing length were recorded in each group,and deltamethrin resistance of the mosquitoes was assessed using larval bioassays and contact tube tests for adults.Results Significant developmental differences were observed across the 3 feeding groups.Larval development time decreased as the food availability increased,and both high-and low-food groups showed reduced pupation rates(χ²=16.282,7.440)and emergence rates(χ²=4.093,6.977)compared to the medium-food group.Adult body weight and wing length were positively correlated with the amount of larval food intake(P<0.05).In high,medium and low food intake groups,larval LC50 values for deltamethrin were 0.110,0.072 and 0.064 mg/L,adult KDT50 values were 97.404,68.964 and 65.005 min,and adult mosquitoe mortality rates at 24 h after deltamethrin exposure were 12%,16%and 19%,respectively.Conclusion The feeding amount during larval stage significantly impacts the development and deltamethrin resistance of Aedes albopictus,suggesting the importance of standardization of larval nutrition for ensuring comparability of resistance test data across laboratories.关键词
白纹伊蚊/抗药性/幼虫食量/溴氰菊酯Key words
Aedes albopictus/insecticide resistance/larval feeding/deltamethrin引用本文复制引用
王颖,邓雯洋,吴超梅,田世焕,李华..白纹伊蚊幼虫摄食量影响生长发育及对溴氰菊酯的抗药性[J].南方医科大学学报,2025,45(3):488-493,6.基金项目
广东省基础与应用基础研究基金(2021A1515011835,2023A1515012133) （2021A1515011835,2023A1515012133）
