南方医科大学学报2025，Vol.45Issue(3)：522-530,9.DOI:10.12122/j.issn.1673-4254.2025.03.10
石墨烯发热膜热疗改善小鼠冻伤的作用及机制
Protective effect of graphene heating film far-infrared hyperthermia against frostbite in mice
摘要
Abstract
Objective To investigate the protective effects of graphene heating film far-infrared(FIR)hyperthermia therapy against frostbite in mice and its impacts on microcirculation and coagulation function.Methods Seventy-six C57BL/6J mice were randomized into control,model,graphene-FIR,and carbon fiber-FIR groups.After 7-day FIR intervention(4 h/day),the mice were subjected to acute(4℃,4 h)and intermittent(4℃,4 h/day for 3 days)cold exposure and the changes in rectal temperature were monitored.In liquid nitrogen frostbite experiment,24 ICR mice were divided into model,graphene-FIR,and carbon fiber-FIR groups,and after a 7-day FIR pretreatment(4 h/day),the liquid nitrogen frostbite models were established and apparent scores of the wounds were assessed on days 3 and 6 after modeling.In carrageenan-induced thrombosis experiment,40 ICR mice were allocated to control,model,graphene-FIR,carbon fiber-FIR,and prazosin groups to test the effect of a 7-day FIR intervention on thrombosis induced by intraperitoneal carrageenan injection(2.5 mg/kg)by measuring thrombus length,blood perfusion,and serum biomarkers(6-keto-PGF1α,TXB2,t-PA,IL-6,IL-1β,TNF-α)24 h after the injection.Results The mice in graphene-FIR group showed significantly elevated rectal temperature in cold exposure tests.In mice with liquid nitrogen-induced frostbite,graphene-FIR treatment significantly reduced the wound scores and reduced frostbite area,producing better effects than carbon fiber.In mice with carrageenan-induced thrombosis,graphene-FIR treatment significantly decreased tail thrombosis length and thrombosis area,increased blood perfusion,lowered serum levels of TXB2,TNF-α and IL-6,and increased the levels of 6-keto-PGF1α and t-PA.Conclusion Graphene heating film FIR therapy can alleviate frostbite injury in mice by improving microcirculation,suppressing thrombosis and inflammatory responses,and reducing coagulation dysfunction.关键词
石墨烯发热膜/远红外/冻伤/微循环/炎症Key words
graphene heating film/far-infrared therapy/frostbite/microcirculation/inflammation引用本文复制引用
张金水,李硕,魏栋栋,成昕,邓云,张有志..石墨烯发热膜热疗改善小鼠冻伤的作用及机制[J].南方医科大学学报,2025,45(3):522-530,9.基金项目
安徽理工大学医学专项培育项目(YZ2023H2C015) （YZ2023H2C015）
国家自然科学基金(81274117) Supported by National Natural Science Foundation of China(81274117). （81274117）
