南方医科大学学报2025，Vol.45Issue(3)：531-541,11.DOI:10.12122/j.issn.1673-4254.2025.03.11
槲皮素通过调控L型钙通道改善糖尿病大鼠心肌损伤
Quercetin ameliorates myocardial injury in diabetic rats by regulating L-type calcium channels
摘要
Abstract
Objective To investigate the effects of quercetin on cuproptosis and L-type calcium currents in the myocardium of diabetic rats.Methods Forty SD rats were randomized into control group and diabetic model groups.The rat models of diabetes mellitus(DM)induced by high-fat and high-sugar diet combined with streptozotocin(STZ)injection were further divided into DM model group,quercetin treatment group,and empagliflozin treatment group(n=10).Blood glucose and body weight were measured every other week,and cardiac function of the rats was evaluated using echocardiography.HE staining,Sirius red staining,and wheat germ agglutinin(WGA)analysis were used to observe the changes in myocardial histomorphology,and serum copper levels and myocardial FDX1 expression were detected.In cultured rat cardiomyocyte H9c2 cells with high-glucose exposure,the effects of quercetin and elesclomol,alone or in combination,on intracellular CK-MB and LDH levels and FDX1 expression were assessed,and the changes in L-type calcium currents were analyzed using patch-clamp technique.Results The diabetic rats exhibited elevated blood glucose,reduced body weight,impaired left ventricular function,increased serum copper levels and myocardial FDX1 expression,decreased L-type calcium currents,and prolonged action potential duration.Quercetin and empagliflozin treatment significantly lowered blood glucose,improved body weight,and restored cardiac function of the diabetic rats,and compared with empagliflozin,quercetin more effectively reduced serum copper levels,downregulated FDX1 expression,and enhanced myocardial L-type calcium currents in diabetic rats.In H9c2 cells,high glucose exposure significantly increased myocardial expressions of FDX1,CK-MB and LDH,which were effectively lowered by quercetin treatment;Elesclomol further elevated FDX1,CK-MB and LDH levels in the exposed cells,and these changes were not significantly affected by the application of quercetin.Conclusion Quercetin ameliorates myocardial injury in diabetic rats possibly by suppressing myocardial cuproptosis signaling and restoring L-type calcium channel activity.关键词
槲皮素/糖尿病心肌病/L-型钙电流/铜死亡Key words
quercetin/diabetic cardiomyopathy/L-type calcium currents/cuproptosis引用本文复制引用
孙红燕,陈睿旸,王磊,康品方,唐碧,卢国庆,付程文,徐梦文,朱小翌,邢国权,刘乐强,柯雨菲,崔乐妹..槲皮素通过调控L型钙通道改善糖尿病大鼠心肌损伤[J].南方医科大学学报,2025,45(3):531-541,11.基金项目
国家自然科学基金(81970313) （81970313）
安徽省自然科学基金(2208085MH192) （2208085MH192）
安徽省临床医学研究转化项目(202304295107020087) （202304295107020087）
安徽省高校协同创新项目(GXXT-2020-019) （GXXT-2020-019）
安徽省高校优秀青年项目(2022AH030141) （2022AH030141）
蚌埠医学院研究生科研创新计划(Byycxz23040) Supported by National Natural Science Foundation of China(81970313). （Byycxz23040）
