赣南医科大学学报2025，Vol.45Issue(2)：107-116,10.DOI:10.3969/j.issn.1001-5779.2025.02.001
酪氨酸氨基转移酶基因在肝细胞癌中诊疗价值及其底物酪氨酸的孟德尔随机化研究
Prognostic value of tyrosine aminotransferase in hepatocellular carcinoma and Mendelian randomization study of its substrate-tyrosine
摘要
Abstract
Objective:To utilize GEO,TCGA and STRING datasets to investigate the clinical value and potential function of tyrosine aminotransferase gene(TAT)in hepatocellular carcinoma(HCC).Methods:The correlation between TAT gene expression and prognosis,clinical stage and immune cell infiltration in HCC patients was analyzed using databases such as GEO,TCGA and STRING and online tools such as GEPIA,and molecules that directly interact with TAT were obtained.Immunohistochemistry(IHC)staining confirmed the expression of TAT in HCC.GO and KEGG enrichment was performed to analyze the potential role of TAT in HCC.The causality between TAT substrate(tyrosine)and HCC was analyzed by Mendelian randomization of two samples.Results:IHC confirmed that TAT was significantly under-expressed in HCC samples.Co-expression and differential analysis yielded 11 co-expressed genes,which are mainly involved in amino acid metabolism.Among them,PCK1,SDS,BHMT,CYP2A6 and CTH could directly interact with TAT.Survival analysis showed that patients with low expression of TAT,CTH or BHMT had significantly lower overall survival.It was found that miR-212 had a significant effect on TAT expression.Analysis of immune cell infiltration revealed 10 immune cell types with significant differences between high and low TAT expression groups.Two-sample Mendelian analysis revealed that TAT may not inhibit HCC directly by regulating tyrosine levels.Conclusion:TAT can be used as a diagnostic marker for HCC,providing a new theoretical basis for the diagnosis and treatment of HCC.关键词
酪氨酸氨基转移酶/肝细胞癌/孟德尔随机化Key words
Tyrosine aminotransferase gene/Hepatocellular carcinoma/Mendelian randomization分类
医药卫生引用本文复制引用
李雅菲,隋丽娜,宋承桂,郭添福,刘志平..酪氨酸氨基转移酶基因在肝细胞癌中诊疗价值及其底物酪氨酸的孟德尔随机化研究[J].赣南医科大学学报,2025,45(2):107-116,10.基金项目
国家自然科学基金项目(31960163) （31960163）
江西省自然科学基金项目(20212BAB206003) （20212BAB206003）
