南方医科大学学报2025，Vol.45Issue(3)：566-576,11.DOI:10.12122/j.issn.1673-4254.2025.03.14
CEACAM6通过调控上皮间质转化抑制鼻咽癌细胞的增殖和迁移
CEACAM6 inhibits proliferation and migration of nasopharyngeal carcinoma cells by suppressing epithelial-mesenchymal transition
摘要
Abstract
Objective To investigate CEACAM6 expression in nasopharyngeal carcinoma(NPC)and its regulatory effects on tumor cell proliferation,migration,and epithelial-mesenchymal transition(EMT).Methods CEACAM6 expression in NPC was analyzed using GEO datasets and validated by immunohistochemistry in NPC tissues and by Western blotting and RT-qPCR in NPC cell lines(HNE1,C666-1,HK1,5-8F and CNE2Z)and normal nasopharyngeal epithelial NP69 cells.In the NPC cell lines,the effects of lentivirus-mediated CEACAM6 overexpression and knockdown on cell proliferation,migration,invasion and cytoskeletal structures were evaluated using CCK-8 assay,Edu staining,wound healing assay,Transwell assay,and phalloidin staining.Western blotting was performed to determine the expressions of EMT-related proteins(FN1,ITGA5,ITGB1,E-cadherin,N-cadherin and vimentin)in the NPC cells and the effect of FN1 overexpression on ITGA5 and ITGB1 protein expressions.Results Analysis of the data from the GEO datasets suggested that CEACAM6 was significantly downregulated in NPC,which was associated with poor patient prognosis.Immunohistochemistry also showed low expressions of CEACAM6 in clinical NPC tissues(P<0.05).In NPC cells,CEACAM6 overexpression significantly suppressed cell proliferation,migration and invasion and reduced the fluorescence intensity of actin.CEACAM6 overexpression also resulted in significant downregulation of FN1,ITGA5,ITGB1,N-cadherin and vimentin expressions and upregulation of E-cadherin expression,and FN1 overexpression obviously attenuated the inhibitory effect of CEACAM6 overexpression on ITGA5 and ITGB1 expressions.Conclusion CEACAM6 inhibits NPC cell migration and invasion by inhibiting EMT via regulating FN1,ITGA5 and ITGB1 expressions.关键词
CEACAM6/鼻咽癌/迁移/上皮间质转化Key words
CEACAM6/nasopharyngeal carcinoma/migration/epithelial-mesenchymal transition引用本文复制引用
陶露,韦卓利,王月月,项平..CEACAM6通过调控上皮间质转化抑制鼻咽癌细胞的增殖和迁移[J].南方医科大学学报,2025,45(3):566-576,11.基金项目
安徽省高等学校科学研究项目(2022AH051481) （2022AH051481）
蚌埠医学院自然科学重点项目(2021byzd048) （2021byzd048）
安徽省自然科学基金青年项目(2008085QH404) （2008085QH404）
