分析化学2025，Vol.53Issue(3)：338-345,8.DOI:10.19756/j.issn.0253-3820.241441
基于超声可连续进样的完整单细胞代谢组学质谱分析平台的构建
Construction of A Single-cell Metabolomics Mass Spectrometry Analysis Platform Enabling Continuous Injection Based on Ultrasound
摘要
Abstract
Single-cell metabolite analysis at the small molecule level reveals intercellular heterogeneity and molecular diversity,especially living cell metabolite analysis which can provide more accurate biochemical information.In this study,a comprehensive single-cell metabolomics mass spectrometry analysis platform was constructed based on continuous ultrasonic sample introduction,aiming to improve the utilization rate of single cells and the efficiency of mass spectrometry detection.This platform utilized mechanical motion generated by a miniaturized ultrasound module,which minimally affected cell integrity and viability,enabling cell suspension and dispersion for up to 60 min,with cell viability exceeding 70%.By comparing cell suspension densities and the cell number of mass spectrometry detections between static and ultrasound groups,the results showed that the ultrasound treatment significantly reduced cell sedimentation rate and increased single-cell mass spectrometry detection efficiency.Applying this platform to single-cell analysis of cell line of mouse cerebellar astrocytes(C8D1A)and mouse glioma(GL261)cells achieved clustering and differential analysis of different cell types,demonstrating the method's potential in analyzing cellular heterogeneity and identifying cells.This approach promised to provide new insights and solutions for single-cell analysis.关键词
单细胞/质谱分析/超声/窄内径毛细管/代谢物分析Key words
Single-cell/Mass spectrometry/Ultrasound/Narrow bore capillary/Metabolite analysis引用本文复制引用
张文美,郭晓凯,许太林,郭广生,汪夏燕..基于超声可连续进样的完整单细胞代谢组学质谱分析平台的构建[J].分析化学,2025,53(3):338-345,8.基金项目
国家自然科学基金项目(Nos.22127805,22206008)资助. Supported by the National Natural Science Foundation of China(Nos.22127805,22206008). （Nos.22127805,22206008）
