分析化学2025，Vol.53Issue(3)：346-355,10.DOI:10.19756/j.issn.0253-3820.251029
基于Fe2O3/氮掺杂碳纳米材料类氧化酶活性的抗坏血酸比色检测
Colorimetric Detection of Ascorbic Acid Based on Oxidase-like Activity of Fe2O3/Nitrogen-doped Carbon Nanomaterials
摘要
Abstract
Ferric oxide/nitrogen-doped carbon nanomaterials(Fe2O3/N-C)with high oxidase-like activity were successfully synthesized via the wet chemistry and pyrolysis method using pyrrole and 1,2,3,4-butanetetracarboxylic acid as raw materials and ferric chloride as the oxidant.The structure and morphology of Fe2O3/N-C were characterized by the techniques including scanning electron microscopy,surface scanning elemental analysis,X-ray diffraction,X-ray photoelectron spectroscopy,and Fourier transform infrared spectroscopy.It was revealed that Fe2O3/N-C could efficiently catalyze the conversion of colorless 3,3',5,5'-tetramethylbenzidine(TMB)into blue-colored oxidized TMB(oxTMB).Based on the principle that ascorbic acid(AA)could inhibit the catalytic color-development reaction of Fe2O3/N-C on TMB,resulting in a paler color and a reduction in the absorbance of the system,a colorimetric sensor for sensitive and accurate detection of AA was constructed.The linear range of the sensor for AA detection was 0.25-30.0 μmol/L,and the detection limit was 0.1 μmol/L.Moreover,it was successfully applied to determination of AA in beverage and tablet samples with satisfactory results.关键词
抗坏血酸/类氧化酶/比色传感器/纳米酶Key words
Ascorbic acid/Oxidase mimic/Colorimetric sensor/Nanozyme引用本文复制引用
周欢,罗浩,童宇,庄欠粉,王勇..基于Fe2O3/氮掺杂碳纳米材料类氧化酶活性的抗坏血酸比色检测[J].分析化学,2025,53(3):346-355,10.基金项目
国家自然科学基金项目(No.32160600)、赣鄱俊才支持计划—主要学科学术和技术带头人培养项目(No.20232BCJ22017)、江苏大学现代农业装备与技术教育部重点实验室开放基金(No.MAET202311)和江西省研究生创新专项资金项目(No.YC2023-S035)资助. Supported by the National Natural Science Foundation of China(No.32160600),the Ganpo Juncai Support Plan-Training Project for Academic and Technical Leaders of Major Disciplines in Jiangxi Province(No.20232BCJ22017),the Open Funding of the Key Laboratory of Modern Agricultural Equipment and Technology(Jiangsu University),Ministry of Education(No.MAET202311),and the Special Fund for Graduate Innovation in Jiangxi Province(No.YC2023-S035). （No.32160600）
