分析化学2025，Vol.53Issue(3)：356-363,8.DOI:10.19756/j.issn.0253-3820.251013
以鲁米诺/过氧化氢作为报告体系的微通道电化学发光传感器用于四环素的检测
Microchannel-based Electrochemiluminescence Sensor for Tetracycline Detection Using Luminol/Hydrogen Peroxide as Reporter System
摘要
Abstract
A microchannel-based electrochemiluminescence(ECL)sensor was developed for detection of tetracycline(TC)utilizing luminol/H2O2 as ECL reporting system.The low excitation potential of luminol/H2O2 effectively mitigated the impact of clamping voltage,thereby enhancing the detection performance of the microchannel-based ECL sensor.The microchannel modified with TC aptamer selectively recognized and captured target TC.The positively charged TC reduced the surface charge density within the microchannel,thereby increasing the ionic current in the microchannel,leading to change of ECL signal of system.The experimental conditions such as electrolyte concentration,TC-aptamer concentration,and reaction time between TC and TC-aptamer were optimized.Under optimal conditions,the difference of ECL signal in the absence and presence of TC(∆ECL)exhibited a good linear relationship with TC concentration in the range from 1.00 ng/mL to 200 ng/mL,with a detection limit as low as 0.69 ng/mL.The sensor had good selectivity and was successfully used in detection of TC in milk samples.关键词
电化学发光/鲁米诺/微通道/四环素/适配体Key words
Electrochemiluminescence/Luminol/Microchannel/Tetracycline/Aptamer引用本文复制引用
黄少坤,谢秀琳,蔡华宾,黄艳玲,林悦,林振宇..以鲁米诺/过氧化氢作为报告体系的微通道电化学发光传感器用于四环素的检测[J].分析化学,2025,53(3):356-363,8.基金项目
国家自然科学基金项目(No.22374024)和福州大学化学学院化学拔尖人才创新训练计划项目(No.HXBJ202404)资助. Supported by the National Natural Science Foundation of China(No.22374024)and the Innovative Training Program for Chemistry Top Talents in College of Chemistry,Fuzhou University(No.HXBJ202404). （No.22374024）
