耕作与栽培2025，Vol.45Issue(2)：19-23,30,6.
不同遮荫处理的红花龙胆代谢组学研究
Metabolomics Study on Gentiana rhodantha with Different Shading Treatments
摘要
Abstract
This study aims to reveals the differences in metabolites of Gentiana rhodantha under differ-ent light treatments,analyzes its main metabolic pathways,and screens for key differential metabo-lites involved in light stress.The results showed that a total of 1 298 metabolites could be detected,of which 196 differentially expressed components were detected in MS45 vs MS0,53 were upregulated,and 143 were downregulated.A total of 165 differentially expressed components were detected in MS90 vs MS0,with 59 upregulated and 106 downregulated.A total of 90 differentially expressed components were detected in MS90 vs MS45,with 57 upregulated and 33 downregulated.The KEGG enrichment analysis showed that there were 6 identical enrichment pathways in the three comparison groups,with 4 significantly enriched.Flavonoids accounted for 48％of the total detected metabolites,while flavonoids and flavonols accounted for 67.4％of the total flavonoids.Flavonoids and flavonols were important differential metabolites in response to light differences.The differences in the number of metabolites in G.rhodantha under different light treatments,mainly were influenced by the accu-mulation patterns of upregulation in flavonoids and flavonols,which were key substances involved in the response of G.rhodantha to strong light stress.Metabolomics methods could quickly and effec-tively analyze the differences in metabolites in G.rhodantha,which was the response the changes in metabolites and physiological ecological under different light treatments,and could provide references for the cultivation of G.rhodantha.关键词
红花龙胆/代谢组分析/黄酮和黄酮醇类Key words
Gentiana rhodantha/metabolomics analysis/flavonoids and flavonols分类
农业科技引用本文复制引用
叶青青,吴之坤,任得强..不同遮荫处理的红花龙胆代谢组学研究[J].耕作与栽培,2025,45(2):19-23,30,6.基金项目
贵州省科技计划项目(黔科合支撑[2023]一般468) （黔科合支撑[2023]一般468）
贵州省科技计划项目(黔科中引地[2022]4016) （黔科中引地[2022]4016）
