分析化学2025，Vol.53Issue(3)：418-428,11.DOI:10.19756/j.issn.0253-3820.241493
大肠杆菌胞外多糖/万古霉素联用抗耐甲氧西林金黄色葡萄球菌生物膜性能分析
Escherichia coli Exopolysaccharides/Vancomycin Combination for Combating Methicillin-resistant Staphylococcus aureus Biofilm
摘要
Abstract
Biofilm formation is one of the key reasons that bacterial infections are difficult to treat.So it is of great significance to develop effective strategies to resist bacterial biofilms.Although antibiotics are important clinical tools for the treatment of bacterial infections,their therapeutic efficacy is often unsatisfactory when targeting bacterial biofilm-associated diseases.In this study,exopolysaccharides(EPS)from Escherichia coli(E.coli)were extracted and purified.It was demonstrated that the obtained E.coli EPS had the ability to inhibit methicillin-resistant Staphylococcus aureus(MRSA)biofilm formation and disperse a mature biofilm.To improve the anti-biofilm effect of vancomycin(VAN),E.coli EPS was used in combination with VAN.The combination increased the inhibition rate of MRSA biofilm and increased the dispersion rate of mature biofilm from 10%to 80%.When combined with E.coli EPS,the number of bacterial colonies within the MRSA biofilm remarkably decreased by 88%,resulting in a significant improvement over the use of VAN alone at an equivalent concentration.Meanwhile,E.coli EPS could down-regulate the expression of MRSA biofilm-related genes.E.coli EPS showed good anti-biofilm effect,and E.coli EPS/VAN combination could provided a potential strategy for treatment of MRSA biofilm infections.关键词
生物膜/大肠杆菌胞外多糖/耐甲氧西林金黄色葡萄球菌/抗生素/生物膜Key words
Biofilm/Escherichia coliexopolysaccharides/Methicillin-resistant Staphylococcus aureus/Antibiotics/Biofilm引用本文复制引用
万晨潇,鞠晓燕,田野,牛忠伟..大肠杆菌胞外多糖/万古霉素联用抗耐甲氧西林金黄色葡萄球菌生物膜性能分析[J].分析化学,2025,53(3):418-428,11.基金项目
国家自然科学基金项目(Nos.52073293,82204326,52273160)和中国科学院理化技术研究所所长基金资助. Supported by the National Natural Science Foundation of China(Nos.52073293,82204326,52273160)and the Presidential Foundation of Technical Institute of Physics and Chemistry,Chinese Academy of Sciences. （Nos.52073293,82204326,52273160）
