临床与病理杂志2024，Vol.44Issue(11)：1475-1487,13.DOI:10.11817/j.issn.2095-6959.2024.240723
基于TCGA数据库研究CXCR4基因在胃癌中的表达及其对肿瘤微环境的影响
CXCR4 gene expression and its impact on the tumor microenvironment in gastric cancer based on TCGA database
摘要
Abstract
Objective:The chemokine family is involved in various physiological and pathological processes including immune regulation,inflammation,and tissue development.C-X-C chemokine receptor type 4(CXCR4)plays a guiding and regulatory role in the tumor microenvironment.This study aims to explore the expression of the CXCR4 gene in the gastric cancer microenvironment and its clinical significance. Methods:Gastric cancer transcriptome data and clinical information were obtained from The Cancer Genome Atlas(TCGA)database.The ESTIMATE algorithm and DESeq2 were used to analyze differential expression of CXCR4 gene and its association with clinicopathological parameters.Surgical gastric cancer specimens collected from Hefei First People's Hospital between August 2022 and August 2023 were analyzed using reverse transcription-polymerase chain reaction(RT-PCR)and immunohistochemistry to validate gene expression.The CIBERSORT algorithm was applied to evaluate the correlation between CXCR4 expression and immune cell infiltration. Results:TCGA data showed significantly higher CXCR4 gene expression in tumor tissues compared to adjacent non-tumor tissues(P<0.001).CXCR4 expression was significantly associated with tumor invasion depth and distant metastasis(P<0.05),and positively correlated with tumor metastasis and degree of differentiation(P<0.05).Kaplan-Meier survival analysis using both TCGA and clinical data showed that high CXCR4 expression was associated with shorter survival time(P=0.003,P<0.001,respectively).Immune cell infiltration analysis revealed positive correlations between CXCR4 expression and memory B cells as well as CD8+T cells(all P<0.05),while negative correlations were observed with resting memory CD4+T cells,activated dendritic cells,plasma cells,activated nature killer cells,M0 macrophages,and activated mast cells(all P<0.05). Conclusion:High expression of CXCR4 in gastric cancer is associated with poor prognosis and closely linked to tumor progression and metastasis.Its expression is also related to immune cell infiltration,suggesting that CXCR4 may serve as a potential target for immunotherapy.关键词
胃癌/趋化因子受体4/临床特征/肿瘤微环境/免疫标志物Key words
gastric cancer/C-X-C chemokine receptor type 4/clinical features/tumor microenvironment/immune markers引用本文复制引用
张新哲,余超,朱广玉,熊伟..基于TCGA数据库研究CXCR4基因在胃癌中的表达及其对肿瘤微环境的影响[J].临床与病理杂志,2024,44(11):1475-1487,13.基金项目
安徽省中医药传承创新科研项目(2024CCCX084).This work was supported by the Medical Research Projects of Anhui Provincial Administration of Traditional Chinese Medicine,China(2024CCCX084). （2024CCCX084）
