胡国敏 1赵杨 2符雪如 2吴钰莹 2卢洁 2张红艳3
作者信息
- 1. 南阳市第一人民医院儿二科 河南南阳 473000
- 2. 郑州大学公共卫生学院流行病与卫生统计学系 郑州 450001
- 3. 郑州大学第三附属医院保健部 郑州 450052
- 折叠
摘要
Abstract
Aim:To explore anti-leukemic efficacy of nuclear factor kappa B(NF-κB)inhibitor parthenolide(PN)in combination with daunorubicin(DNR)in mice with myeloid leukemia.Methods:Twenty nude mice were used to construct xenograft model by inoculating acute monocytic leukemia THP-1 cells and randomly assigned into blank control group(trea-ted with PBS),PN group,DNR group,and PN+DNR group,5 mice for per group.The mice in PN group were treated with 0.2 μg PN per mouse,intraperitoneal injected once every 2 days within 14 days.The mice in DNR group were treated with 0.6 mg DNR,injected on day 1,2,3,8,9 and 10.The mice in PN+DNR group were treated with combination of PN and DNR,using the same treatment regimen as above.All mice were executed on day 16 and tumors were separated and weigh-ted,and the anti-tumor rate in each group was calculated.Cell apoptosis in the separated tumor tissue was tested using TUNEL assay.Results:On day 16,the changes of tumor volumes of blank control group,PN group,DNR group,and PN+DNR group were(1 365.13±426.79),(695.68±129.29),(117.68±58.36),and(-44.55±78.74)mm3,respec-tively;both PN and DNR could inhibit tumor volume,and there was a synergistic effect when they were combined(P=0.034).The tumor inhibition rates of PN group,DNR group,and PN+DNR group were 32.5％,71.9％,and 86.8％,re-spectively.The cell apoptosis rates of the above groups were(4.50±1.58)％,(13.20±3.36)％,(34.32±3.40)％,and(36.62±3.56)％,respectively;both PN and DNR could induce the apoptosis of tumor cells,and there was a synergistic effect between them(P=0.038).Conclusion:PN has anti-myeloid leukemia effects,and PN and DNR have a synergistic effect in combination.关键词
髓系白血病/核因子κB/parthenolide/柔红霉素/裸鼠/移植瘤Key words
myeloid leukemia/nuclear factor kappa B/parthenolide/daunorubicin/nude mouse/xenograft分类
临床医学
