河南农业大学学报2025，Vol.59Issue(2)：233-242,10.DOI:10.16445/j.cnki.1000-2340.20240527.001
胡杨PeMAX2对拟南芥镉吸收和耐受能力的影响
Effects of Populus euphratica PeMAX2 on cadmium uptake and tolerance in Arabidopsis
摘要
Abstract
[Objective]This study investigated the physiological and molecular mechanisms of MAX2(MORE AXILLARY BRANCHES2)in Populus euphratica in the regulation of plant response to cad-mium stress,which is of great significance for the mining,screening and identification of key genes related to heavy metal tolerance.[Method]Arabidopsis thaliana overexpressing PeMAX2(PeMAX2-OE1 and PeMAX2-OE2),wild-type(WT)and Atmax2 mutant were used as experimental materials,the role of PeMAX2 in regulating cadmium tolerance in plants was determined by using seven-day-old seedlings grown on 1/2 MS medium and 30-day-old soil-cultured seedlings.[Result]The relative elec-trical conductivity of the PeMAX2-overexpressed lines were significantly lower than those of the WT and the Atmax2 mutants in Arabidopsis thaliana seedlings after cadmium treatment,which is due to the improved antioxidant capacity.The superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)activities of the transgenic lines increased remarkably,which was significantly higher than that of WT and Atmax2.Under cadmium stress,the cadmium-altered transcription of the antioxidant enzymes resembles the pattern of enzyme activity.In addition,the chlorophyll content of the trans-genic line decreased less than that of WT and Atmax2 after being treated CdCl2 for 10 days of soil-cultured Arabidopsis thaliana seedlings.The photosynthetic and chlorophyll fluorescence parameters of transgenic lines were higher than those of WT and mutants.[Conclusion]The overexpression of P.euphratica PeMAX2 gene increased cadmium uptake in Arabidopsis thaliana,and transgenic plants improved Cd tolerance by increasing antioxidant enzyme activity.关键词
胡杨/PeMAX2/拟南芥/重金属/镉胁迫/光合作用/抗氧化酶Key words
Populus euphratica/PeMAX2/Arabidopsis thaliana/heavy metals/cadmium stress/photosynthesis/antioxidant enzyme分类
生物学引用本文复制引用
安珂悦,马思圆,李静,赵瑞,陈少良,赵楠,周晓阳,尧俊..胡杨PeMAX2对拟南芥镉吸收和耐受能力的影响[J].河南农业大学学报,2025,59(2):233-242,10.基金项目
国家自然科学基金项目(32371828,32071730,317706643) （32371828,32071730,317706643）
高等学校学科创新引智计划项目(111Project,B130007) （111Project,B130007）
北京市自然科学基金项目(6182030,6172024) （6182030,6172024）
中央高校基本科研业务专项(2019ZY25) （2019ZY25）
