猪圆环病毒2型灭活疫苗的植物源免疫增强剂筛选OA北大核心

[Objective]The aim of this study was to screen a safe and efficient immune booster against porcine circovirus type 2 vaccine,and to improve the immune efficacy and safety of existing adjuvants.[Method]In this study,Kunming mice were selected as the research object,and a total of 16 plant-based active substances were selected:terpenoids(artemisinin,taxol,andrographolide,ursolic acid),alkaloids(berberine,lycorine,cepharanthine,oxymatrine),phenolic acids(caffeic acid,chlorogenic acid,gallic acid,coumaric acid)and flavonoids(kaempferol,quercetin,luteolin,proanthocyanidin).By measuring the body mass ratio,IgG antibody level,organ index,total superoxide dismutase(T-SOD),malondialdehyde(MDA)and total antioxidant capacity(T-AOC)of mice,the immune enhance-ment agents targeting porcine circovirus type 2 inactivated vaccine were screened and the antioxidant activity was evaluated.[Result]1)The body mass of mice increased after the administration of plant derived active substances.2)The antibody levels of all groups showed a trend of first increasing and then decreasing after vaccination.Compared with vaccine group,berberine,lycorine,cepharanthine,quercetin,luteolin and proanthocyanidin groups could significantly improve the antibody level of the body,and the proanthocyanidin group had the best effect,among which the antibody level was increased by 53.59%.3)The results of organ index displayded that the heart,liver and kidney indexes in querce-tin group were significantly increased,but there were no significant differences in berberine,lycorine and proanthocyanidin groups.The spleen and thymus indexes of quercetin and proanthocyanidin groups were significantly increased by 49.50%and 53.23%,121.82%and 103.64%,suggesting that querce-tin may play an important role in promoting the development,differentiation and maturation of immune organs and improving the immune function of the body.4)The T-SOD activity in luteolin,quercetin and proanthocyanidin groups was significantly increased,MDA content was significantly decreased,and T-AOC content was significantly increased in cepharanthine,lycorine,quercetin,luteolin,berberine and proanthocyanidin groups.The antioxidant effect of proanthocyanidins was better,T-SOD activity and T-AOC were increased by 9.60%and 48.57%,respectively,and MDA was decreased by 26.82%.[Con-clusion]Proanthocyanidins have the best immune enhancement effect on mice,and could be used as a candidate for the study of porcine circovirus type 2 inactivated vaccine adjuvant.