河南农业大学学报2025，Vol.59Issue(2)：255-265,11.DOI:10.16445/j.cnki.1000-2340.20240722.001
鲤RNF14基因的生物学功能及其对病原刺激的免疫响应
Biological function of RNF14 gene in Cyprinus carpio and its immune response to pathogen stimulation
摘要
Abstract
[Objective]This study aims to explore the biological function of ring finger protein 14 gene(RNF14)in Cyprinus carpio,and to provide new ideas for blocking spring viremia of Cyprinus carpio virus(SVCV)infection.[Method]The function of RNF14 gene in Cyprinus carpio was preliminarily analyzed by PCR,bioinformatics analysis and real-time fluorescence quantitative PCR,and its expre-ssion pattern after virus stimulation was studied.[Result]RNF14 gene in Cyprinus carpio was 1 362 bp in length,encoding 453 amino acids.The RNF14 protein of Cyprinus carpio had closer relationship with Sinocyclocheilus rhinocerous and farther relationship with Columba livia.The protein is an acidic unstable hydrophilic protein;Functional domain prediction showed that the protein was a typical RBR E3s.The results of ubiquitination substrate prediction showed that the protein interacted with RBCK1,Nedd4l,Notch1a and other proteins with higher confidence.Tissues expression profile analysis showed that RNF14 gene was widely expressed in various tissues or organs,with the highest expression in the heart.Compared with the control group,the mRNA expression level of RNF14 gene was signifi-cantly increased in muscle at 24 h after high-dose SVCV infection.The mRNA expression level of RNF14 gene was significantly increased in kidney and head kidney after 24 h of Ploy(I:C)stimula-tion in vivo.Compared with the control group,the mRNA expression level of RNF14 gene in Cyprinus carpio was significantly increased at each sampling time point after low-dose SVCV and Ploy(I:C)infection in vitro.The results of pathogen stimulation in vivo and in vitro showed that the RNF14 gene of Cyprinus carpio was involved in the immune response process after pathogen stimulation,suggesting that the RNF14 gene of Cyprinus carpio may be a potential target for blocking SVCV infection.[Con-clusion]The CDS region of RNF14 gene in Cyprinus carpio was successfully cloned,and the immune response of RNF14 gene in Cyprinus carpio under SVCV and Ploy(I:C)stimulation was revealed.关键词
鲤/环指蛋白14/生物信息学/鲤春季病毒血症病毒/多肌胞苷酸/病原刺激/免疫响应Key words
Cyprinus carpio/ring finger protein 14/bioinformatics analysis/spring viremia of Cypri-nus carpio virus(SVCV)/polyinosinic acid-polycytidylic acid/pathogenic stimulation/immune response分类
水产学引用本文复制引用
张美娜,王延晖,刘变枝,张子豪,洪佳乐,郭朝辉,李海洁,于光晴,董鹏生,黄小城,杨振江..鲤RNF14基因的生物学功能及其对病原刺激的免疫响应[J].河南农业大学学报,2025,59(2):255-265,11.基金项目
国家自然科学基金项目(31702359) （31702359）
河南省科技研发联合基金项目(222103810017) （222103810017）
河南省高等学校重点科研项目(23A240001) （23A240001）
河南省现代农业产业技术体系(HARS-22-16-T) （HARS-22-16-T）
