河南农业大学学报2025，Vol.59Issue(2)：266-275,10.DOI:10.16445/j.cnki.1000-2340.20240412.001
酶法提取头孢菌素C菌渣中蛋白质工艺优化
Optimization of enzymatic extraction of protein from cephalosporin C fermentation residues
摘要
Abstract
[Objective]To study the process conditions for the efficient extraction of proteins from cephalosporin C residues by enzyme method,expanding the resource utilization of antibiotic residues.[Method]Under the optimum conditions of six enzymes,lysozyme,snail enzyme,acidic protease,neutral protease,alkaline protease and papain,the protein of cephalosporin C residues was extracted,and the optimum enzyme was determined used on the protein extraction rate;The effects of enzyme addition mass fraction,temperature,time,pH value and V/mL∶m(cephalosporin C residues/g)(liquid-solid ratio)on the extraction of the protein in cephalosporin C residues were investigated,and the optimum conditions of extraction were determined by optimization of the response surface test.[Result]Lysozyme was the optimum enzyme for protein extraction from cephalosporin C fermentation residues.The protein extraction efficiency was mainly affected by three factors in the order of liquid-solid ratio,time and pH value.The optimal process conditions for protein extraction were as follows:liquid-solid ratio of 7.0∶1.0,extraction time of 99 min,pH value 7.2,enzyme amount of 1.0%,tem-perature of 35℃,and the highest protein extraction rate of 69.06%was achieved under this condi-tion.[Conclusion]Lysozyme is suitable for enzymatic hydrolysis and comprehensive utilization of cephalosporin C fermentation residues,which can quickly and effectively extract the proteins from bac-terial fermentation residues.关键词
头孢菌素C/抗生素菌渣/溶菌酶/蛋白质提取/资源化利用Key words
cephalosporin C/fermentation residues/lysozyme/protein extraction/resource utilization分类
农业科学引用本文复制引用
赵会如,刘柯妤,张立美,田甜甜,张继冉,徐淑霞,张世敏,吴坤..酶法提取头孢菌素C菌渣中蛋白质工艺优化[J].河南农业大学学报,2025,59(2):266-275,10.基金项目
河南省重点研发专项(221111112300) （221111112300）
河南省科技攻关项目(222102110120) （222102110120）
河南农业大学高层次人才专项支持基金项目(30501469) （30501469）
