河南农业大学学报2025，Vol.59Issue(2)：276-284,9.DOI:10.16445/j.cnki.1000-2340.20240628.001
高温胁迫对棉铃虫齿唇姬蜂胚胎发育的影响
Effects of high temperature stress on embryonic development of Campoletis chlorideae
摘要
Abstract
[Objective]Evaluate the effect of global warming on the pest control potential of the dominant natural enemy Campoletis chlorideae in China,the effects of high temperature stress on the embryonic development of this wasp were investigated.[Method]The parasitized host Helicoverpa armigera larvae were reared at constant high temperature of 32,35,36 and 37℃,the parasitized larvae were dissected regularly,and photographed using stereomicroscope or phase contrast micro-scope to observe the embryonic development states and characteristics of C.chlorideae.Compared with those at 25℃,the embryonic development duration,encapsulation rate,survival rate and developmental volume of the wasp embryos were calculated and analyzed.[Result]At 35℃,the embryonic development duration of C.chlorideae was 1.78 d,which was significantly longer than that at 32℃(1.65 d),but shorter than that at normal temperature(2.04 d),indicating that 35℃had an adverse effect on embryonic development.Compared with normal temperature,high temperature conditions significantly increased the encapsulation rate of embryos,decreased the sur-vival rate,and exerted an obvious lethal effect on embryos.Under high temperature stress at 37℃,the encapsulation rate increased by 9.76 times compared to room temperature.This result showed that the immune encapsulation response of Helicoverpa armigera to wasp embryos induced by high temperature increased continuously.The lethal temperature for the embryonic development of this wasp was 36℃,at which the larvae could not hatch,but the embryos could develop to some extent.There was significant difference in the tolerance to high temperature between two different strains,the embryonic development of F2 from the field strain was significantly superior to that of F15 laboratory strain.[Conclusion]The high temperature threshold is 35℃for the embryonic development of C.chlorideae,while temperatures≥36℃has a lethal effect on the embryo.Using embryonic development as a critical developmental period,the negative effects of high temperature on parasitic wasps can be quickly detected.关键词
高温/棉铃虫齿唇姬蜂/胚胎发育/棉铃虫/包囊/致死Key words
high temperature/Campoletis chlorideae/embryonic development/Helicoverpa armigera/encapsulation/lethal effect分类
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谷晓行,杨成都,李昊泊,魏纪珍,李欣,白素芬..高温胁迫对棉铃虫齿唇姬蜂胚胎发育的影响[J].河南农业大学学报,2025,59(2):276-284,9.基金项目
河南省高等学校重点科研项目(25B210011) （25B210011）
