河南农业大学学报2025，Vol.59Issue(2)：285-294,10.DOI:10.16445/j.cnki.1000-2340.20241111.001
甲硫氨酸富集蛋白在棉铃虫卵巢发育中的功能及机制解析
Functional mechanism of methionine-rich storage protein in the ovarian development of Helicoverpa armigera
摘要
Abstract
[Objective]To investigate the function and mechanism of Methionine-rich storage protein(HaSP1)in the ovarian development of Helicoverpa armigera.[Method]The effects of protein shortage on ovarian development from four-instar larvae,HaSP1 transcriptional expression,and juvenile hor-mone(JH)titer were analyzed.The open reading frame of HaSP1 was amplified by PCR and subjected to bioinformatics analysis.The transcriptional expression profile of HaSP1 in the ovaries and fat bodies of females from three days before emergence to four days after emergence was measured using real-time quantitative polymerase chain reaction(qRT-PCR).Finally,the effects of HaSP1 knockdown in females'ovarian development,total amino acid content,methionine content,and HaVg transcrip-tional expression were analyzed using RNA interference(RNAi).[Result]Protein deficiency in the larval stage significantly inhibited the reproductive capacity of females and led to markedly decreases in JH titer and HaSP1 transcription.The open reading frame of HaSP1 is 2 256 bp,and HaSP1 is more closely to SP1 in Mythimna loreyi,and Trichoplusia ni.HaSP1 was highly expressed in ovaries on the second day before emergence,and the expression peak in fat body occurred on the third day before emergence.Knocking down HaSP1 inhibited ovarian development and significantly reduced HaVg transcription,total amino acid content,and methionine content.[Conclusion]HaSP1 played an important role in the ovarian development of H.armigera by affecting methionine content.关键词
棉铃虫/甲硫氨酸富集蛋白SP1/卵巢发育/RNA干扰/甲硫氨酸Key words
Helicoverpa armigera/methionine-rich storage protein SP1/ovarian development/RNA interference/methionine分类
农业科技引用本文复制引用
秦千越,方斌,王靖康,张博,耿梓宸,安世恒,李祥,赵文丽..甲硫氨酸富集蛋白在棉铃虫卵巢发育中的功能及机制解析[J].河南农业大学学报,2025,59(2):285-294,10.基金项目
国家自然科学基金项目(31801339,32302345) （31801339,32302345）
河南省自然科学基金青年项目(232300420188) （232300420188）
河南省高等学校重点科研项目(23A210005) （23A210005）
