郑州大学学报（医学版）2025，Vol.60Issue(2)：149-153,5.DOI:10.13705/j.issn.1671-6825.2024.03.040
艾迪注射液抗HepG2裸鼠移植瘤的作用及对胆碱代谢通路的影响
Anti-tumor effect of Aidi injection on HepG2 transplanted tumor in nude mice and its effect on choline metabolism pathway
摘要
Abstract
Aim:To investigate the anti-tumor effect of Aidi injection(ADI)on hepatocellular carcinoma(HCC)HepG2 transplanted tumor in nude mice and its effect on choline metabolism pathway.Methods:HepG2 cells(1 × 106 cells/mL)were inoculated at 0.1 mL per mouse into the right axilla of nude mice.When the volume of the transplanted tumors reached 50 mm3,the nude mice were randomly allocated into 2 groups,with 6 mice in each group:one group received intraperitoneal injections of 20 mL/kg normal saline(model group),and the other group received 20 mL/kg ADI(ADI group),and the injections were administered every other day.After 8 administrations,the mice were euthanized,and the tumor volumes were measured.Serum was collected for untargeted metabolomics analysis to identify differential metabolites associated with the cancer choline metabolism pathway.The protein and mRNA expression levels of CHKα in tumor tissue were detected by Western blot and RT-qPCR.Results:The tumor volume in the ADI group was significantly smaller than that in the model group(P＜0.05).Serum metabolomics analysis revealed significant differences in metabolite profiles be-tween the 2 groups,with 2 differential metabolites enriched in the cancer choline metabolism pathway:1,2-dihexadecanoyl-sn-glycero-3-phosphocholine(DPPC)and 1-stearoyl-2-arachidonoyl-sn-glycerol(1,2-SAG).The expression levels of both metabolites were lower in the ADI group compared with the model group(P＜0.05).Additionally,the protein and mRNA expression levels of CHKα in tumor tissue in the ADI group were significantly lower than those in the model group(P＜0.05).Conclusion:ADI exerts its inhibitory effect on the progression of HCC by downregulating the expression of CHKα,thereby suppressing choline metabolism in tumor cells.关键词
肝细胞癌/艾迪注射液/胆碱激酶A/非靶代谢组学/裸鼠Key words
hepatocellular carcinoma/Aidi injection/choline kinase A/non-targeted metabolomics/nude mouse分类
临床医学引用本文复制引用
费炳红,于东升,郭丹风,李晓萍..艾迪注射液抗HepG2裸鼠移植瘤的作用及对胆碱代谢通路的影响[J].郑州大学学报（医学版）,2025,60(2):149-153,5.基金项目
国家自然科学基金青年基金项目(81900468,81901571) （81900468,81901571）
河南省重点研发与推广专项(222102310534) （222102310534）
河南省慈善联合总会肝胆相照基金项目(GDXZ2023022) （GDXZ2023022）
