郑州大学学报（医学版）2025，Vol.60Issue(2)：162-166,5.DOI:10.13705/j.issn.1671-6825.2024.02.040
人脐带间充质干细胞移植对未成熟小鼠脑白质损伤的影响
Effect of human umbilical cord mesenchymal stem cell transplantation on white matter injury in immature mice
摘要
Abstract
Aim:To investigate the effect of human umbilical cord mesenchymal stem cells(hUC-MSC)transplantation on white matter injury in immature mice.Methods:P5 C57BL/6J mice were allocated into 4 groups of 27 each.The normal control group was not treated;in the sham operation group,the common carotid artery was not transected;in the model group and MSC transplantation group,the injury model was induced by transecting the common carotid artery.The MSC transplan-tation group received 5 x 105 hUC-MSC injection into the lateral ventricle post-surgery.The model group received PBS in-stead.On day 7 post-surgery,7 mice from each group were randomly chosen and underwent MRI to assess brain injury,fol-lowed by immunohistochemical and immunofluorescence staining to analyze the expressions of microtubule-associated protein 2(MAP2)and myelin basic protein(MBP)which marking the cerebral grey matter and white matter,as well as brain tissue neurons,astrocytes,and microglia.From day 24 post-surgery,the remaining 20 mice began to undergo neurobehavioral tests,including novel object recognition,Y-maze experiment,rotarod and CatWalk gait analysis.And 7 mice were taken from each group to assess the development of white matter using DTI 120 days after surgery and fractional anisotropy(FA)was as-sessed.Results:In comparison with the model group,the MSC transplantation group exhibited significant reductions in cere-bral infarction volume,white matter loss volume ratio,and gray matter loss volume ratio(P＜0.05).The percentage of astro-cytes in the hippocampus was significantly lower in the MSC transplantation group[(10.3±1.3)％]compared with the model group[(15.7±1.3)％](P＜0.05).The MSC transplantation group also demonstrated significant increase in the spontaneous alternation rate,recognition index and rotarod-turning time(P＜0.05).Compared with the model group(0.191±0.002),the FA of the MSC transplantation group(0.224±0.004)was elevated(P＜0.05).Conclusion:hUC-MSC could alleviate the degree of white matter injury in immature mice,enhance white matter development,and improve the long-term learning memory and motor function deficits,and its neuroprotective effect may be related to inhibiting the activation of as-trocytes.关键词
人脐带间充质干细胞:未成熟脑/脑白质损伤/缺氧缺血/行为学/小鼠Key words
human umbilical cord mesenchymal stem cell/immature brain/white matter injury/hypoxia-ischemia/e-thology/mouse分类
医药卫生引用本文复制引用
张含,宋娟,王军,李文冬,彭茜瑞,杨禄祥,张新玲,曹洋洋..人脐带间充质干细胞移植对未成熟小鼠脑白质损伤的影响[J].郑州大学学报（医学版）,2025,60(2):162-166,5.基金项目
河南省医学科技攻关计划项目(LHGJ20210453) （LHGJ20210453）
